分子生物学英文版教学课件:Chapter 6 Translation.ppt,This is a picture of the yeast 80S ribosome. We can see the numerous proteins mixed with rRNA strands. 这是一张酵母80S核糖体的图片,能够从中看到无数的蛋白质与rRNA混杂在一起。 Secondly, eukaryotes use a
Rareineukaryotes,butverycommoninprokaryoticcellularandviralmRNAs.SomeeukaryoticmRNAscontainupstreamAUGcodonsthatterminatebeforethedownstreamreadingframe.Theupstreamopenreadingframesmaybetranslated,withreinitiationoccurringatthedownstreamopenreadingframe. IninfluenzaBvirusmRNA,M2initiationcodonispartoftheterminationcodonforM1pr...
3. SsrA is a 457-nt tmRNA Prokaryotes: Fig 14-39 SsrA rescues the stalled ribosomes degrade mRNAs that are incomplete or have premature stop codons Eukaryotes 1.Nonsense mediated mRNA decay 2.Nonstop mediated decay When an mRNA contains 44、 a premature stop codon (nonsense codon), the ...
Translation In Eukaryotes Differences between the two systems 1) Translation transcription are not coupled 2) Ribosomes are larger. 3) The initiating amino acid is still Met, but it is not formylated. 4) Eukaryotic mRNA is capped. This is used as the recognition feature for ribosome binding ...
Translation-翻译 EukaryoticmRNATranscriptsareProcessed DNA Transcription RNA RNAProcessing mRNAG AAAAAA Nucleus 2021/4/4 Nuclearpores Cytoplasm G Export AAAAAA 1 A“Simple”EukaryoticGene TranscriptionStartSite 5’UTR Introns 3’UTR 5’Exon1Int.1Exon2Int.2Exon3 3’Promoter/ControlRegion Exons Transcrip...
RNA component of the Ribosome The RNA component in the ribosome acts as a ribozyme! It catalyzes the peptidyl transferase reaction which forms peptide bonds between two adjacent amino acids Prokaryotes The 23S RNA component in the 50S ribosome subunit Eukaryotes The 28S RNA component in the 60S ...
AUGUAAAUGUAAInprokaryotes,ribosomescanfindthesecondstartcodontotranslatetheoverlappinggenewithoutdetachingfromthetemplate.EukaryotestendtomakeuseofalternativeRNAprocessing.tRNAs:adoptermoleculestRNAaretheadoptermolecules(转接分子)thatdeliveraminoacidstotheribosomeanddecodetheinformationinmRNA.1PrimarystructureoftRNALinear...
(Lostin)Translation 1.Brieflyreviewprokaryoticmachinery 2.InitiationinEukaryotes 3.WhereintheworldisPeptidyl Transferase? 4.tRNAcharging:Thesecondcode 1968NobelPrizeinPhysiology&Medicine (fordecipheringthegeneticcode) “TriumphoftheChemists” H.G.KhoranaR.HolleyM.Nirenberg Usedacell-freeproteinsynthesissystemfrom...
G. Regulation of translation initiation in eukaryotes: mechanisms and biological targets. Cell 136, 731–745 (2009). 8. Le Bacquer, O. et al. Elevated sensitivity to diet-induced obesity and insulin resistance in mice lacking 4E-BP1 and 4E-BP2. J. Clin. Invest. 117, 387–396 (2007)....
in the C-terminal extension between ICT1 and C12orf65 (Supplementary Fig.1), implying different interactions with ribosomes. As in the case forICT1, genes homologous toC12orf65are found in most eukaryotes; however, in contrast toICT1, they have not yet been detected in bacteria. The study...