Several optimizations were made on this original protocol but the effect of vibration or rotational shaking (revolution per minute) accompanied by the heat shock has not been studied. This paper reports the effect of shaking during or after the heat shock of competent DH5-Alpha strain of E. ...
Application PCR technology, with the opposite sex increased the lead sheep pox virus structural protein 2 gene, BKLP pMD connected to L8-T - Carrier, converted to the feeling state DH 5 Alpha cells of the reorganization, the dual-enzyme all plasmids BKLP 2 gene, will be connected to a pe...
Lactic acid bacteria (LAB) are one of the microorganisms of choice for the development of protein delivery systems for therapeutic purposes. Although there are numerous tools to facilitate genome engineering of lactobacilli; transformation efficiency sti
Total RNA was extracted from the transformation systems after exposure to 5 mg/L of ibuprofen, naproxen, gemfibrozil, diclofenac, propranolol or iopromide. Extractions were done using the RNeasy Mini Kit (QIAGEN®, Germany) except the protocol included an extra bead-beating step for cell ...
The LIM and SH3 domain protein 1 (Lasp1) was originally cloned from metastatic breast cancer and characterised as an adaptor molecule associated with tumourigenesis and cancer cell invasion. However, the regulation of Lasp1 and its function in the aggres
The precipitation kinetics of NbC carbide during and after gamma/alpha transformation of a Nb microalloyed steel was investigated experimentally. The mole fractions of NbC precipitates formed at different isothermal temperatures were measured as a function of holding time by using the electrical ...
The protocol of the motivational theory stipulates that, in this final phase, all inquiries or responses to the participant should be presented in a positive manner, without judgment or negativity. The questions of the motivational interviewing technique are presented in Appendix B. The questions, ...
tumefaciens-mediated genetic transformation protocol was developed. Keywords: 16srRNA; EHA 105; GUS; kanamycin; LBA 4404 1. Introduction Plants have been used by humans for food, flavouring, healthcare and a variety of other purposes since ancient times. Many civilizations’ ancient written records ...
Palladium precatalysts for decarbonylative dehydration of fatty acids to linear alpha olefins. ACS Catal. 2016, 6, 7784–7789. [CrossRef] 13. Goossen, L.J.; Rodriguez, N. A mild and efficient protocol for the conversion of carboxylic acids to olefins by a catalytic decarbonylative elimination...