Saunders JJ, Docherty A, Humphreys GO (1984) Transformation of bacteria by plasmid DNA. In: Bennet PM, Grinsted J (eds) Methods in microbiology, vol 17 London: Academic Press, pp 61–95Saunders, J.R., Docherty, A. and Humphreys, G.O. (1984) Transformation of bacteria with plasmid DNA...
SECTION 8-1 – Transformation of BacteriaThis chapter explains the procedure for transforming bacterial cells with circular DNA plasmids containing an origin of autonomous DNA replication and a selectable marker. Logarithmically, growing cells are made permeable to DNA by incubation in CaCl 2 , ...
competent cells ever take up DNA, one of the problems facing geneticists is how to identify and keep the rare transformed cells, while getting rid of the rest. The most common solution to this problem is to use plasmids. A plasmid is ...
b.The introduction of DNA from one cell into another by means of a bacteriophage or one of a variety of chemical or physical methods. trans′for·ma′tion·al,trans·form′a·tive(-fôr′mə-tĭv)adj. American Heritage® Dictionary of the English Language, Fifth Edition. Copyright ...
Prior to transformation, recombinant plasmids are constructed by inserting the DNA sequence of interest into a vector as described in traditional cloning basics. In transformation, the DNA, typically in the form of a plasmid, is in...
A simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. The basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) DNA on cellphone membranes placed successively on nutrient and selective agar. Using...
We have been considering the steps necessary to produce genetically engineered bacteria capable of producing human insulin. First, we inserted the human insulin gene into a bacterial plasmid using restriction enzymes and ligase. Then, we inserted this recombinant plasmid into the bacteria E. coli...
Lactic acid bacteria (LAB) are one of the microorganisms of choice for the development of protein delivery systems for therapeutic purposes. Although there are numerous tools to facilitate genome engineering of lactobacilli; transformation efficiency still limits the ability to engineer their genomes. Whi...
This chapter reviews the discovery, development, and establishment of DNA-mediated transformation as a way for bacteria to exchange genetic material. The processes of natural competence development, DNA uptake, and recombination of the incoming DNA strand are discussed in detail for the Streptococcus pn...
Abstract Genetic engineering requires a procedure for introducing DNA into host cells, followed by integration into the host genome and gene expression. Although several procedures for DNA-medi-ated gene transfer in mammalian cells, yeast and bacteria have been reported, no such methods are yet avail...