Zahm P, Hohmeyer C, Geider K: Site-specific mutagenesis of the Ti plasmid by transformation of Agrobacterium tumefaciens with mutagenized T-DNA fragments cloned in E. coli plasmids. Mol Gen Genet 194: 188-194, 1984.Zahm, P, Hohmeyer, C, Geider, K (1984) Site-specific mutagenesis of ...
Early vectors used for transformation were closely based on wild-type strains ofAgrobacteriumand were deletion derivatives ofTi plasmids(co-integrative vectors tended to be large and were problematic in enabling efficientplant regenerationand for assembling the transgene constructs). It was found that the...
Ti plasmid can be used as a vector for transformation involvingAgrobacterium tumefaciensand is used for producing transgenic plants. With the use of restriction enzymes, a particular gene of interest can be inserted into the plasmid and transformed. It is used for the development of stress-tolerant...
The present study aims to investigate transformation of Agrobacterium tumefaciens GV3101 with the pSoup plasmid isolated from Escherichia coli DH5α by electroporation. The maximum transformation frequency was 0.21x10-6 transformants/ Ug DNA achieved at field strength of 19.0KV/cm with pulse of 2.0mse...
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Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmiddoi:10.1111/j.1365-2443.2012.01612.xAgrobacterium strains containing a Ti plasmid can transfer T-DNA not only to plants but also to fungi, ...
中文: 用发根农杆菌(Agrobacterium rhizogenes)的Ri质粒转化药用植物牛膝(Achyranthes bidentataBl.) ,诱导出了抗卡那霉素的发状根。更详细... 英文: Among thirty strains of the leu diazotroph Enterobacter cloacae E in the rice rhizosphere transformed with the plasmid pMC7A LEU, only one strain E can be...
英文: Tomato Transgenatic System with Ti Plasmid of Agrobacterium tumefaciens中文: 番茄外源基因转化系统的研究 英文: PRELIMINARY STUDY OF TRANSFORMATION OF TI PLASMID IN CARROT CELL中文: Ti质粒转化胡萝卜细胞的初步研究 英文: TRANSFERRING CHITINASE GENE INTO WHEATWITH TI-PLASMID BINARY VECTOR SYSTEM中文:...
20 November 2015 Nopaline-type Ti plasmid of Agrobacterium encodes a VirF-like functional F-box protein Benoît Lacroix & Vitaly Citovsky During Agrobacterium-mediated genetic transformation of plants, several bacterial virulence (Vir) proteins are translocated into the host cell to facilitate in...
During Agrobacterium-mediated genetic transformation of plants, several bacterial virulence (Vir) proteins are translocated into the host cell to facilitate infection. One of the most important of such translocated factors is VirF, an F-box protein produced by octopine strains of Agrobacterium, which ...