(siRNA)into a cell, the presence of having a fast, easy-to-use method to optimize transfection conditions for given cell lines would be of great value.Three to four siRNA sequences are generally tested for each gene inorder to suppress the expression of a protein with a correspondingmRNA ...
siRNACationic lipidRNAi screeningAssayRNAi screening of mammalian cells is often performed using siRNAs and cationic lipids as transfection reagents. Efficiency of transfection depends on growth characteristics of the cells and the cationic lipid used. With a large selection of cationic lipids available, ...
Powerful--performs with low cytotoxicity in the presence or absence of serum Streamlined Transfection Protocol siRNA transfection is traditionally a two-day procedure, making optimization a relatively slow process. siPORT NeoFX Transfection Agent was developed to refine siRNA transfection ...
Optimization of siRNA transfection into Human Umbilical Vein Endothelial Cells and Aortic Smooth Muscle Cells for the knockdown of zinc finger protein 496 expression A Teklu 被引量: 0发表: 2011年 加载更多来源期刊 Endothelium 1997/02/01 研究点推荐 Transfection Human Endothelial Cells transfection metho...
Simple protocol for easy optimization in a broad spectrum of cell lines Watch the siRNA transfection protocol video Resources With greater knockdown at lower siRNA concentrations, Lipofectamine RNAiMAX outperforms other reagents When it comes to achieving effective gene knockdown, Lipofectamine RNAiMAX T...
10.Effect of ultrasound combined with contrast agent on efficiency of VEGF gene transferring into fibroblasts in vitro超声联合超声造影剂对VEGF基因体外转染成纤维细胞转染率的影响 11.OPTIMIZATION OF TRANSFECTION CONDITIONS FOR TRANFECTING TF SIRNA TO HEK-293 CELLS MEDIATED BY CATIONIC LIPOSOME优化阳离子脂...
Quick and easy protocol & optimization Request a free sample today to test in your lab! LEARN MORE NEW!– Superior and Gentle siRNA Transfection FuGENE®SI: Superior siRNA delivery Maximizes gene silencing Minimal-to-no cytotoxicity Request a free sample today to test in your lab!
siRNA Transfection Why use the Xfect RNA Transfection Reagent to transfect siRNA oligos? It's effective—get better knockdown than with the leading competitor It's easy—no optimization necessary It's nontoxic—Xfect won't kill your cells
Fig. 13.Schematic diagram showing the steps of nanoparticle–DNA binding in ss-DNA or siRNA delivery process including membrane binding, cellular entry, endosomal escape, DNA release and nuclear trafficking. Nanostructured carbon materials have been widely used as scaffolds or vectors for drug delivery...
After 24 h, cells were treated with Ar plasma for 40 s and 20 μL of DNA-FITC (0.8 μg), siRNA (2.0 μg), miRNA (2.0 μg) or pEGFP-C1 (0.4 mg) was added to the plate for plasma transfection. After another 24 h, 10 mL RPMI medium was mixed...