How it Works: Transfecting B-cell Lymphomas With siRNABioRad Laboratories
This cation channel was customized to mammalian cells, proving its functionality by transfecting neurons and exposing them to blue-light (470 nm), which induced polarization by admitting sodium in Nagel et al. (2003) and Boyden et al. (2005). This was followed by the Natronomonas ...
Some cells, like HeLa cells, are easy totransfectwith lipid reagents. Others, like stem cells, primary neurons and unstimulated T and B cells, not so much. For these,electroporationhas become the method of choice, with researchers resorting to more involved methods, such asviral transduction...
In early 1990s, direct expression of external mRNA molecules in host animals for therapeutic effects was first tested by delivering RNA vectors encoding a reporter gene such as luciferase and β-galactosidase into murine muscle cells and transfecting vasopressin mRNA into rats to reverse Diabetes-Insip...
If natural sources of high-density ion channels are unavailable, recombinant proteins can be overproduced by transfecting heterologous expression systems with vectors that incorporate powerful promoters. Methods range from using yeast and bacterial systems, such as Pichia pastoris and Escherichia coli, to...
Needle-free biolistic transfection as mediated by gene gun [69] and PMED (particle-mediated epidermal delivery [236] devices transfers microparticle-adsorbed DNA into the epidermal layer by helium force to transfect LC, dermal DC (and keratinocytes). Of note, the physical stress associated with ...