siRNA transfection is a powerful tool used to understand the mechanisms of gene regulation and molecular pathways. The following 10 tips will help you to optimize your siRNA transfection.
How it Works: Transfecting B-cell Lymphomas With siRNABioRad Laboratories
This cation channel was customized to mammalian cells, proving its functionality by transfecting neurons and exposing them to blue-light (470 nm), which induced polarization by admitting sodium in Nagel et al. (2003) and Boyden et al. (2005). This was followed by the Natronomonas ...
of double-stranded RNA molecules into the cell, usually by transfection. Double-stranded siRNA can be simulated by a vector-based shRNA approach. Lentivirus-based vectors may be suitable because they are able to infect target cells with high efficiency and are, in general, safe to use. ▪ ...
If natural sources of high-density ion channels are unavailable, recombinant proteins can be overproduced by transfecting heterologous expression systems with vectors that incorporate powerful promoters. Methods range from using yeast and bacterial systems, such as Pichia pastoris and Escherichia coli, to...
of shRNA driven by RNA-Pol III-dependent promoters (H1 or U6) in the absence of tetracycline, which is two tetracycline operons that need to be placed flanking the TATA box [35,36]. Corresponding lentiviral systems have also been developed for cells that are difficult to transfect [34]. ...
To transfect as many APCs as possible, targeting DNA vaccine delivery to secondary lymphoid organs via systemic application like intravenous injection [213], oral [214] or pulmonary [215] administration is a suitable strategy. Alternatively, DNA vaccines are often applied often topically via the skin...