3.1. Raw read processing and de novo assembly The illumina sequencing run generated a total of 56,321,907 paired end reads of 101 bp length consisting of 11.3 Gb nucleotides (Accession: PRJNA287587 ID: 287587). The quality check showed the average base quality was above Q20 (error-probabi...
Transcriptome sequencing and assembly represent a great resource for the study of non-model species, and many metrics have been used to evaluate and compare these assemblies. Unfortunately, it is still unclear which of these metrics accurately reflect assembly quality. Results We simulated sequencing t...
Summary of the transcriptome sequencing and assembly.Santosh, Kumar Upadhyay
3.1. Transcriptome sequencing and assembly We characterized the visceral mass transcriptome of a Camaenidae family land snail, S. myomphala, critically endangered in Korea, using the Illumina HiSeq 2500 NGS platform. Each sequencing lane generated 2 × 50 nucleotides independent reads from either en...
Transcriptome sequencing, while getting cheaper, is still an expensive endeavor, with an examination of data quality and its assembly infrequently performed in depth. Here, we outline many of the important issues we think need consideration when starting a transcriptome sequencing project. We also ...
Transcriptome sequencing and assembly Total RNA was isolated from each of the frozen whole-bodies of beetles. mRNA was purified from the total RNA using oligo-dT attached magnetic beads. Following mRNA fragmentation, cDNA synthesis, end repair, 3’ adenylation, adaptor ligation, and PCR enrichment,...
Transcriptome sequencing was performed on three groups of material and three replicates were used to undertake sequencing. Transcriptome sequencing and assembly RNA with Poly-A structure in eukaryotic total RNA was enriched using the TIANSeq mRNA capture kit (TIANGEN Biotech). Nanodrop 2000 ...
Sequencing and assembly As the main immune organ of gastropod mollusks, the hepatopancreas plays an important role in the organism’s resistance to biotic and abiotic threats [37]. Hepatopancreas samples from 12 snails were subjected to transcriptome sequencing and data analysis. The sequencing da...
Sequencing and de novo assembly of chrysanthemum transcriptome Strand-specific RNA-seq libraries were prepared from whole plants of chrysanthemum [Chrysanthemum morifolium(Ramat) Kitamura] cv. Fall Color, under normal and dehydration conditions, respectively. Relative water content (RWC) of leaves was 94....
Transcriptome sequencing and assembly To comprehensively investigate the transcriptome and gene expression profiles of D. grandiflorum under normal and low temperature stress, four cDNA sample from leaves of chrysanthemum seedlings were prepared and sequenced using Illumina HiSeq 4000 platform. An overview ...