cDNA synthesis, end repair, 3’ adenylation, adaptor ligation, and PCR enrichment, four RNA sequencing (RNA-Seq) libraries were constructed using the Illumina TruSeq RNA sample preparation kit. The raw
Through a combination of metabolite analysis with transcriptome sequencing, a new hypothesis was proposed to explain the lack of colour phenotype of the white variant of the blue grape hyacinth. Grape hyacinth (Muscari) is an important ornamental bulbous plant with an extraordinary blue colour. Muscar...
Six libraries (three dark-red and three white) were constructed and analyzed to elucidate differences in cyanidin (Cy) metabolism between dark-red and white onion bulbs. Libraries were screened using RNA-sequencing (RNA-seq) to reveal the differentially expressed genes (DEGs) involved in ...
transcriptome sequencing and de novo analysis for ma bamboo (dendrocalamus latiflorus munro) using the illumina platform:转录组测序和de novo分析麻竹(dendrocalamus latiflorus蒙罗)使用illumina平台 热度: 转录组高通量测序基本知识介绍 热度: 转录组测序数据分析小软件介绍 ...
Introduction to Total RNA Sequencing Whole-transcriptome analysis with total RNA sequencing (RNA-Seq) detects coding plus multiple forms of noncoding RNA. Total RNA-Seq can accurately measure gene and transcript abundance, and identify known and novel features of the transcriptome. ...
In this study, embryonic calli of two maize inbred lines with strong redifferentiation capacity and two lines with weak redifferentiation capability were separately subjected to transcriptome sequencing analysis during the early redifferentiation stages (stage I, 1–3 d; stage II, 4–6 d; stage II...
Gene expression quantification from sequencing data was performed using Cufflinks with the normalized number of fragments per kilobase of exon per million reads. Correlations of mean expression values among groups were calculated. Microarray analysis of additional molar germs from E50, E60 and E70 was...
美[trænsk'rɪptɒm] 英[trænsk'rɪptɒm] 网络转录组;转录体;转录子组 复数:transcriptomes 英英 网络释义 n. 1. thefullcomplementofuniquesequencedRNAmoleculeswithcodedgeneticinformationthatanindividualproduces 例句 释义: 全部,转录组,转录体,转录子组 ...
Genome sequencing and analysis of the model grass Brachypodium distachyon. Nature 463, 763–768 (2010). Ayliffe, M., Singh, D., Park, R., Moscou, M. & Pryor, T. Infection of Brachypodium distachyon with selected grass rust pathogens. Mol. Plant-Microbe Interact. 26, 946–957 (2013). ...
precatorius33, andPogostemon cablin34, have been reported. In addition, the published studies have shown that the obtained transcriptome sequences contain large numbers of EST-SSRs14,35. EST-SSRs developed based on transcriptome sequencing data can be used in the genetic analysis of both a sequence...