Our cloning strategy is specifically designed to densely sample regulatory regions, in order to enable high-resolution inference of regulatory activity from highly overlapping fragments. Indeed, we found up to 370 unique fragments per region in our HiDRA libraries, with ~32,000 genomic intervals conta...
Bioinformatics Genome-wide discovery of cis-regulatory RNA motifs and transcriptional regulatory networks WASHINGTON UNIVERSITY IN ST. LOUIS Gary D. Stormo XuXinggenomes helps extend our knowledge and deepen our understanding on gene regulation at both transcriptional and post-transcriptional levels in ...
RBPs generally bind to specific untranslated regions of the RNA (the regulatory regions, called cis-elements). RBPs are present in all living cells and can bind to more than one messenger RNA with sequence specificity. Such interactions play an important role in regulating target RNA localization...
The present invention provides a novel fructose responsive transcription control cis-element and a transcriptional regulatory factor that interacts therewith, a non-human animal having them transferred or inactivated, a diagnostic method for genetic susceptibility to a metabolic disorder using them, and a...
4a–c). Most of these changes were in intronic or intergenic regions consistent with cis-regulatory or enhancer elements (Extended Data Fig. 7a,b). Fig. 4: BATF3 OE remodels the chromatin landscape in the context of acute or chronic T cell stimulation. a, Number of ATAC-seq regions ...
Finally, we found that upregulated KIAA1199 expression in human breast cancer specimens correlated with hypomethylation of the regulatory region. Involvement of DNA methylation in regulation of KIAA1199 expression was recapitulated in human breast cancer cell lines. Taken together, our study unraveled ...
region is co-precipitated (as measured by PCR), the target locus is likely to bind the protein (directly or indirectly)in vivo. The immunoprecipitated DNA can also be used for hybridization to high-density microarrays (ChIP–chip) or for high-throughput sequencing (ChIP–seq) to provide a ...
The REDfly database of Drosophila transcriptional cis-regulatory elements provides the broadest and most comprehensive available resource for experimentall... SM Gallo,DT Gerrard,M David,... - 《Nucleic Acids Research》 被引量: 295发表: 2010年 Optimization of transcriptional regulatory elements for co...
Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities. Mol. Cell 38, 576–589 (2010). CAS PubMed PubMed Central Google Scholar Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25,...
A 2.8 kb acnA monocistronic transcript was detected by Northern blot hybridization, but only in redox-stressed (methyl-viologen-treated) cultures, and a 2.5 kb acnB monocistronic transcript was detected in exponential- but not stationary-phase cultures. These findings are consistent with previous ...