The most significantly enriched term was ‘transcription, DNA-templated’, with a p value of 9.15 × 10−97. This was followed by biological processes linked to positive and negative regulation of transcription with p values of <2.17 × 10−31. Fig. 3: Hierarchical clustering ...
cell cycle checkpoints, mitotic DNA damage checkpoints and the MCM complex, while it down-regulated apoptosis-related processes such as chromatin assembly, DNA conformation change, apoptotic processes, response to oxidative stress, the inflammatory...
The molecular function 'transcription coregulator activity' is a part of the biological process 'regulation of transcription, DNA-templated' that occurs in the cellular component 'chromatin'. It can mechanistically link sequence-specific DNA-binding transcription factor (dbTF) regulatory DNA target ...
respectively. qRT-PCRs were performed using LightCycler® FastStart DNA MasterPLUS SYBR Green I reaction mix and a LightCycler 2.0 Instrument (Roche, Basel, Switzerland) to determine the relative mRNA levels in each
negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiati...
negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentia...
negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent; visual perc...
有研究利用RNA -Seq来研究体外转录反应产物序列和产量分布情况,发现IVT反应中的RNA产物末端可以折回形成发卡结构,将自身变成引物,在3'端添加NTPs,产生长度超过预期产物的RNA,并且随着RNA产物浓度的升高,此过程会跟以DNA为模板的正常转录过程发生竞争。以上过程,可以看作是独立于启动子序列的聚合酶活性。说白了,就是...
Collisions of the transcription and replication machineries on the same DNA strand can pose a significant threat to genomic stability. These collisions occur in part due to the formation of RNA-DNA hybrids termed R-loops, in which a newly transcribed RNA
Glenn GerardIn eukaryotes, DNA is packaged by histone proteins to form nucleosomes - the fundamental unit of chromatin. Aside from their structural role in DNA compaction, histones are dynamic regulators of DNA accessibility and, hence, are important for DNA-templated processes including transcription...