This chapter describes the methods that have been used to characterize the interaction of trp RNA-binding attenuation protein (TRAP) with RNA, and to study how these interactions are involved in regulating transcription attenuation of the trp operon. TRAP is encoded by mtrB, the second gene of ...
摘要: The evidence leading to the concept of attenuation as a major control system for operons concerned with amino acid biosynthesis is reviewed.关键词: bacteria DOI: 10.1016/0968-0004(81)90066-9 被引量: 3 年份: 1981 收藏 引用 批量引用 报错 分享 ...
Transcription attenuation can also occur by other mechanisms, involving RNA-binding proteins in particular. For instance, the attenuation of thetrpoperon inB. subtilisis mediated by TRAP (fortrpRNA-binding attenuation protein, a toroid-shaped molecule) which acts by binding to the (G/U) AG-repea...
Fig. 1: The overall structures ofMtbPhoP-TAC. aDNA scaffold used in structure determination ofMtb2PhoP-TAC (top panel) and two views of the cryo-EM density map ofMtb2PhoP-TAC (bottom panel).bDNA scaffold used in structure determination ofMtb4PhoP-TAC (top panel) and two views of the ...
* Although the trp operon does not have positive regulation, it does have an extra level of regulation. This extra level of regulation is called attenuation. This mechanism is important because repression by the trp repressor alone is not very strong; transcription still continues at a significant...
Structural studies of antibiotics not only provide a shortcut to medicine allowing for rational structure-based drug design, but may also capture snapshots of dynamic intermediates that become 'frozen' after inhibitor binding. Myxopyronin inhibits bacterial RNA polymerase (RNAP) by an unknown mechanism...
The Bacillus subtilis AT (anti-TRAP) protein inhibits the regulatory protein TRAP (trp RNA-binding attenuation protein), thereby eliminating transcription termination in the leader region of the trp operon. Transcription of the AT operon is activated by uncharged tryptophan transfer RNA (tRNATrp). ...
第五章原核生物的转录调控(Regulationoftranscriptioninprokaryotes)Regulationofgeneexpression:anoverviewThelacoperonThetrpoperonTranscriptionalregulationbyalternativeσfactor 第一节基因表达调控总论 一、基因表达及方式 基因表达(geneexpression)：遗传信息由DNA到蛋白质或功能RNA的过程。根据生物对内外...
Why don't eukaryotes use attenuation to regulate their genes? What are the differences between bacterial and mammalian transcription? Coordinate regulation of gene expression is important both in bacteria and eukaryotes. Operons are used to coordinate gene expression in bacteria but not eukaryote...
F. Mechanism of UTP-modulated attenuation at the pyrE gene of Escherichia coli: an example of operon polarity control through the coupling of translation to transcription. EMBO J. 3, 2857–2861 (1984). Article CAS PubMed PubMed Central Google Scholar Abe, H., Abo, T. & Aiba, H. ...