bytryptophanoperon have gotimprovement. This experiment iS mainlyto improve the enzymeactivity and finallyimprove the production of L—tryptophanbycloning thewhole operon and increasinggene copies.Besides,we also analyzed the self-promoter’S effectin expressing and ...
Gene Expression in Bacterial Systems: The trp Operon and Attenuation - Encyclopedia of Biological Chemistry (Second Edition)Transcription attenuation is defined as any mechanism that utilizes transcriptional pausing or termination to control expression of downstream genes. This mechanism of regulation is ...
The E. coli tryptophan (trp) operon consists of a regulatory region foIldwid by five structural genes ( trp E through trp A) 1,2 . The trp structural gene products, Which are co-ordinately synthesized in equimolar amounts, catalyze the conversion of chorismate to tryptophan 1,3 . Transcri...
Studying the regulation of tryptophan biosynthetic operons has provided insight into fundamental aspects of gene regulation in bacteria. In the model bacterium Escherichia coli, the trpEDCBA operon is regulated by two complementary mechanisms: TrpR- mediated tryptophan-dependent transcriptional repression26 ...
Construction and Properties of Plasmid Vectors Containing the trp Regulatory Region Suitable for Expressing Foreign GenesThe E. coli tryptophan (trp) operon consists of a regulatory region foIldwid by five structural genes ( trp E through trp A) 1,2 . The trp structural gene products, Which ...
coli, Y denotes a sequence of one or more genetically codable amino acids which permits the following amino acid sequence Z to be cleaved off, and Z is a sequence of genetically codable amino acids, characterized by expressing a gene structure which codes for these fusion proteins in a host ...
coli, Y denotes a sequence of one or more genetically codable amino acids which permits the following amino acid sequence Z to be cleaved off, and Z is a sequence of genetically codable amino acids, characterized by expressing a gene structure which codes for these fusion proteins in a host ...
J., Brunel, F. (1974) Quantitative aspects of gene expression in a λ-trp fusion operon. Molec. gen. Genet. 130: pp. 9Davison J, Brammar WJ, Brunel F. Quantitative aspects of gene expression in a lambda-trp fusion operon. Mol Gen Genet. 1974 Apr 9; 130 (1):9–20....
(1991) PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products. Mol. Gen. Genet. 226, 41-48.Hill et al., “PCR based gene engineering of the Vibrio harveyi lux operon and the ...
coli, Y denotes a sequence of one or more genetically codable amino acids which permits the following amino acid sequence Z to be cleaved off, and Z is a sequence of genetically codable amino acids, characterized by expressing a gene structure which codes for these fusion proteins in a host ...