wherein when the trans-splicing ribozyme is expressed in a cell, the catalytic RNA sequence cleaves the mRNA and ligates the donor transcript to the mRNA to generate a spliced mRNA which comprises the donor transcript, such that the donor transcript is translated as part of the spliced mRNA ...
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence ...
A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Harvard obtains United States patent More results ► Medical browser ? ▲ Transracial Adoption transradial...
M. Molecular recognition in a trans excision-splicing ribozyme: non-Watson–Crick base pairs at the 5′ splice site and ωG at the 3′ splice site can play a role in determining the binding register of reaction substrates. Biochemistry 44, 1067–1077 (2005). Article CAS PubMed Google ...
We have targeted trans -splicing ribozymes against mRNAs of chloramphenicol acetyltransferase, human immunodeficiency virus, and cucumber mosaic virus, and demonstrated trans -splicing and delivery of a marker gene in Escherichia coli cells. The improved trans -splicing ribozymes may be tailored for ...
Cell ablation using trans-splicing ribozymes 专利名称：Cell ablation using trans-splicing ribozymes 发明人：James Haseloff,Andrea Brand,Norbert Perrimon,Howard M. Goodman 申请号：US08/482663 申请日：19950607 公开号：US05882907A 公开日：19990316 专利内容由知识产权出版社提供 摘要：The design of new ...
Trans-splicing ribozymes for targeted gene delivery Ribozymes are potential tools for genetic manipulation, and various naturally occurring catalytic RNAs have been dissected and used as the basis for the de... U K?Hler,BG Ayre,HM Goodman,... - 《Journal of Molecular Biology》 被引量: 194发...
Although it is known that some group II introns are capable of self-splicingin vitroand are therefore also referred to asribozymes, protein machinery and other cofactors are required for efficient splicingin vivo(Bonen and Vogel, 2001). InC. reinhardtiiat least 14 nuclear genes are required for...
We have developed and validated a new tumor-targeting gene therapy strategy based upon the targeting and replacement of human telomerase reverse transcriptase (hTERT) RNA, using atrans-splicing ribozyme. By constructing novel adenoviral vectors harboring the hTERT-targetingtrans-splicing ribozymes with th...