trans-amplifying RNA miRNA delivery gene regulation trans-replication Introduction RNA interference (RNAi) is a posttranscriptional regulatory mechanism that uses small noncoding RNAs to direct sequence-specific mRNA degradation or translational repression.1 In mammalian cells, common exogenous RNAi mediators...
“Arcturus”, Nasdaq: ARCT), a global late-stage clinical messenger RNA medicines company focused on the development of infectious disease vaccines and opportunities within liver and respiratory rare diseases, today announced that the U.S. Food and Drug Administration (FDA) has granted Fast Track ...
The GFP fragment (942 bp) was produced by amplifying the pEGFP-C1 plasmid using a polymerase chain reaction in the Proflex PCR system (ThermoFisher Scientific). The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.). In addition, the 40-nt ...
PCRs targeted at amplifying DNA containing the MSY-1-binding site on the Glut 3 gene (-89 to +260 bp) from immunoprecipitated protein complexed to chromatin in the presence of an anti-MSY-1 antibody (MSY-1 Ab, lane 4) revealed a 354-bp region. Negative control consisted of no primary...
Total RNA was extracted from these sections to generate cDNA using RT-PCR. cDNAs were amplified using oligonucleotides specific to human Tau (hTau, 117 base pairs) or murine Tau (70 base pairs). The positive control was prepared by amplifying the plasmid containing hTau sequence. The lower ...
To solve this problem, we employed the same data augmentation tech- nique used in [21] to balance the classes by amplifying the training set's positive samples 20 times. This was achieved by sliding a window with a fixed size from the right or left over the DNA sequences while ensuring ...
To the best of our knowledge, this is the first one-step pan-IAV real-time reverse-transcription PCR which has been validated by amplifying nine IAV However, standard Sanger sequencing being validated with virus isolation, and was used to detect IAV in poultry swabs under clinical diagnostic ...
reaction volume by amplifying β 2 microglobulin (β 2 - MG) C 1 C 2 (60 ◦ C, 30 s) for 30 cycles [10] as control. To determine the best number of cycles, PCR was per- formed from 20 to 37 cycles respectively. The PCR products from each cycle were run on 1% agarose gel,...
Levels of normal and mutant plectin mRNA were measured by semiquantitative real-time PCR (SQRT-PCR), amplifying complementary DNA (cDNA) from wild-type fibroblasts, transfected and non-transfected EBS-MD fibroblasts. A wild-type specific forward PCR primer was designed to discriminate between wild...
We applied it to the design of an electrochemiluminescent (ECL) biosensor to detect the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) gene. We first activated the cleavage activity of CRISPR-Cas12a by amplifying the target DNA into a segment of double-stranded DNA through the amplification ...