such as sickle cell disease homozygous carriers, because of alloimmunization. In vitro production of cultured red blood cells is slowly emerging as a possible complement to the existing collection-based red blood cell procurement
In primary cells, the innate immune response inhibits taRNA expression. We addressed this issue with the same approach that we used to improve gene expression by self-amplifying RNA - cotransfecting viral immune evasion proteins E3 and B18.33 As effective as this method was, it may be possibl...
OTC deficiency is a serious urea cycle disorder with a prevalence of approximately 10,000 people worldwide. A lack of OTC, a critical urea cycle enzyme in liver cells, results in high blood ammonia levels that can cause diminished cognitive ability, seizures, coma and death. The current standa...
Furthermore, focusing within the crypt compartment, we analyzed cells at different levels of differentiation as determined by the EphB2 receptor levels: intestinal stem cells (ISC, EphB2high), transient-amplifying cells (TA, EphB2medium and EphB2low) and differentiated cells (EphB2negative) using...
Full-length untagged rat SALM4 (aa 1–626) expression construct was generated by amplifying the insert from a rat brain cDNA library (BD Bioscience Clontech) by PCR and subcloning it into GW1 vector. Haemagglutinin (HA)-tagged full-length mouse SALM3 (aa 28–636) was subcloned into ...
To solve this problem, we employed the same data augmentation tech- nique used in [21] to balance the classes by amplifying the training set's positive samples 20 times. This was achieved by sliding a window with a fixed size from the right or left over the DNA sequences while ensuring ...
Full-length untagged rat SALM4 (aa 1–626) expression construct was generated by amplifying the insert from a rat brain cDNA library (BD Bioscience Clontech) by PCR and subcloning it into GW1 vector. Haemagglutinin (HA)-tagged full-length mouse SALM3 (aa 28–636) was subcloned into ...
The GFP fragment (942 bp) was produced by amplifying the pEGFP-C1 plasmid using a polymerase chain reaction in the Proflex PCR system (ThermoFisher Scientific). The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.). In addition, the 40-nt ...
Primer sequences for amplifying Myf5 and GAPDH are as described (Pan et al., 2011). Chromatin immunoprecipitation (ChIP) assays ChIP assays were performed with 10T1/2 cells overexpressing FLAG–Zic1 and Pax3–HA using the Fast ChIP method (Nelson et al., 2006) with some modifications. ...
A 2% agarose gel demonstrates PCR amplifications that were performed on immunoprecipitated chromatin from N2A cells. PCRs targeted at amplifying DNA containing the MSY-1-binding site on the Glut 3 gene (-89 to +260 bp) from immunoprecipitated protein complexed to chromatin in the presence of ...