Analyze both coding RNA and multiple forms of noncoding RNA for a comprehensive view of the transcriptome.
Ion Total RNA-Seq 试剂盒 v2 包括在 Ion GeneStudio S5、Ion Proton 或 Ion Personal Genome Machine (PGM) 系统上为链特异性 RNA 测序制备代表性 cDNA 文库所需的试剂。Ion GeneStudio S5 和 Ion Proton 系统是适用于全转录组(去除核糖体 RNA 或 polyA)测序的理想选择,而 Ion GeneStudio S5 和 Ion PGM ...
Since this method does not use a poly(A) selection step we sequence all RNA molecules in a sample including many interesting noncoding RNAs that are usually missed in other conventional assays. As this protocol interrogates the entire length of all RNA molecules in the cell, which includes ...
诺唯赞试剂说明书 RNA-seq 转录组测序技术 VAHTS Total RNA-Seq Library Prep Kit.pdf,NR603-01/02 VAHTSTM Total RNA-seq (H/M/R) ® Library Prep Kit for Illumina Vazyme Biotech Co., Ltd 网站/Web: 咨询热线/Tel: 400-600-9335 w Vazyme biotech co., ltd. w 销售/S
This HMW peak can be reduced by decreasing the number of cycles for PCR 2 to 8 cycles for 10 ng RNA input and 12 cycles for 250 pg input. Switch sequencers without affecting sequence quality RNA-seq data generated on the Singular Genomics G4 Sequencing Platform and Illumina...
NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the co...
RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells are rare. Here, we evaluate the performance of the SMARTer...
total rna library kit simplest library prep prepare ready-to-sequence libraries in as little as 4 hours suitable for ffpe only minimal modifications needed to the standard protocol automation friendly streamlined protocol for increased scalability view product master endless applications with one protocol ...
Next-generation transcriptome sequencing (RNA-Seq) has become the standard practice for studying gene splicing, mutations and changes in gene expression to obtain valuable, accurate biological conclusions. However, obtaining good sequencing coverage and depth to study these is impeded by the difficulties...
Picard tools were used to sort the cleaned SAM files according to their reference sequence names and create an index for the reads. The gene-level expression was quantified using the R packages GenomicFeatures and GenomicRanges. For each cell line, we measured total RNA amount (in ng µl...