抗体名:拓普西异构酶Ⅰ抗体TopoisomeraseI免疫标记 浓度:1mg/ml % 靶点:细胞核,细胞膜等 宿主:Rabbit、mouse 用途:交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 ...
Topo I protein expression was detected in 127 (51%) samples, including 24.4% with >50% positive tumor cells. The majority had nuclear (70.1%) or nuclear and cytoplasmic staining (17.3%). A higher percentage of cells expressing topo I in primary colorectal cancer was significantly associated ...
However, whether topotecan alters synaptic protein levels and synapse function is currently unknown. Here we report that in primary cortical neurons, topotecan depleted synaptic proteins that are encoded by extremely long genes, including Neurexin-1, Neuroligin-1, Cntnap2, and GABA(A)β3. Topotecan...
Chronic hyperglycemia leads to the development of diabetes-induced organ complications, through changes in gene expression and protein function. We previously showed that in cell lines, topoisomerase I (topo I) is modified by O-GlcNAcylation, which affects its DNA relaxation activity. Since topo I ...
PURPOSE: Human DNA topoisomerases I and II (topo-I and -II) are essential for vital cellular processes such as DNA replication, transcription, translation, recombination, and repair. In the present study, we correlate topo-I and -II expression and outcome after chemotherapy in primary and relap...
In the present work, we investigated the role of BTBD1, a protein with an unknown function, which binds to Topo1.28 This protein was detected in humans in 2001 by Carim-Todd et al.29 along with its homologue BTBD2, which shares 82% identity with the BTBD1 amino-acid residue sequence. ...
Drug-induced proteinassociated DNA breaks were also studied as a function of the cell cycle in 3T3 cells that had been arrested by contact inhibition in medium containing 1% calf serum and then stimulated to proliferate by replating at a lower cell density in medium containing 10% serum. In ...
R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops in the human genome, here, we map RNA:DNA hybrids, replication stress markers and DNA double-strand breaks (DSBs) in cells depleted for Topoisomerase I (To
Protein A/G 保存液 PBS, pH 7.4, with 0.05% BSA 内含物 0.05% sodium azide 保存条件 4° C 运输条件 Ambient (domestic); Wet ice (international) 产品详细信息 Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires...
(Tdp1)isoneoftherepairenzymesforTop1-DNAcovalentcomplexes.Tdp1formsamultiproteincomplexthatincludespoly(ADP)ribosepolymerase(PARP).PARP-deficientcellsarehypersensitivetocamptothecinsandfunctionallydeficientforTdp1.WewillreviewrecentdevelopmentsinseveralpathwaysinvolvedintherepairofTop1cleavagecomplexesandtheroleofChk1...