大家好,本周分享一篇发表在Nature Chemical Biology上的文章,题目为“Native top-down proteomics enables discovery in endocrine-resistant breast cancer”,通讯作者是来自Scripps研究所的John Yates教授和Kendall Nettles教授,研究方向分...
top‐down proteomicslarge ions efficient fragmentationcomplementary fragment massescandidate protein sequenceSummary This chapter contains sections titled: Separation of intact proteins Ionization of intact proteins Resolution and accuracy requirements for charge state determination and mass calculation Fragmentation ...
Why HRAM mass spectrometry for top-down proteomics High-resolution accurate mass (HRAM) MS is essential for top-down proteomics. HRAM MS resolves both co-eluting intact proteins as well as isotopic peaks of highly charged, large intact proteins, enabling b...
Many bottom-up proteomics research endeavors also benefit from high-throughput top-down mass spectrometry analysis. The main advantages of the top-down approach include detection of degradation products and sequence variants, which helps to solve inference...
质谱技术是蛋白质组学(proteomics)研究中的关键技术。在蛋白质质谱领域,主要有两种分析策略:Top-down(自上而下)和Bottom-up(自下而上)。这两种方法在处理和分析蛋白质样品时有本质上的不同。 1、Top-down策略(自上而下): 在Top-down方法中,整个蛋白质直接被引入到质谱仪中,并在质谱仪内部被解离为较小的片段...
质谱技术是蛋白质组学（proteomics）研究中的关键技术。在蛋白质质谱领域，主要有两种分析策略：Top-down...
文章Top-down Proteomics of 10,000 Single Brain Cells,介绍了一种自上而下(top-down)的 “单细胞蛋白质成像质谱”(single-cell Proteoform imaging Mass Spectrometry,scPiMS)新方法,能在单个细胞水平上高通量、高分辨率地进行蛋白质组学研究,可以使研究者们以每天1000个或更...
Wright EP, Partridge MA, Padula MP, Gauci VJ, Malladi CS, Coorssen JR (2014) Top-down proteomics: enhancing 2D gel elec- trophoresis from tissue processing to high-sensitivity protein detec- tion. Proteomics 14:872-889Wright EP, Partridge MA, Padula MP, Gauci VJ, Malladi CS, Coorssen ...
In our 2024 webinar, leading researchers in the cutting-edge field of top-down proteomics, will discuss the some of the latest exciting developments around the world. The webinar series has been designed to appeal not only to researchers but also to participants who want to learn about top-dow...
内容提示: “自顶向下 (top-down)”的蛋白质组学*——— 蛋白质变体的规模化鉴定孙瑞祥**罗 兰迟浩刘超 贺思敏(中国科学院智能信息处理重点实验室, 中国科学院计算技术研究所,北京 100190;中国科学院大学,北京 100049)摘要 高分辨率质谱技术的快速发展使得“自顶向下”的蛋白质组学(top-down proteomics)研究逐渐...