FLOW cytometryTUMOR necrosis factorANTHRAXThis study describes a four-color flow cytometric assay that detects CD4+ T cell responses to the anthrax vaccine. Whole blood from seven volunteers who previously obtained the anthrax vaccine was inoculated in vitro with varying concentrations of the anthrax ...
关键词: T cells FLOW cytometry TUMOR necrosis factor ANTHRAX DOI: 10.1016/j.jim.2003.07.012 被引量: 9 年份: 2003 收藏 引用 批量引用 报错 分享 全部来源 免费下载 求助全文 全文购买 Elsevier Elsevier (全网免费下载) Semantic Scholar 掌桥科研 dx.doi.org 查看更多 相似文献 参考文献 引证文献...
Flow cytometry showed that cerebral pericytes express high levels of α5 integrin, and lower levels of α1, α2, and α6 integrins. The pro-inflammatory cytokine tumor necrosis factor (TNF)-α strongly promoted pericyte proliferation and migration, and concomitantly induced a switch in pericyte ...
Since LPS induces internalization of TLR421, we tested cell surface levels of TLR4 by flow cytometry. In THP-1-derived macrophages, tmTNF-α mAb markedly promoted a LPS-induced decline in TLR4 levels on the cell surface within 60 min of stimulation (Fig.5a) but did not affect TLR produc...
Prussin C, Metcalfe DD (1995) Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods 188:117–128 Article PubMed CAS Google Scholar Li MH, Seatter SC, Manthei R, Bubrick M, West MA (1994) Macrophage endotoxin tolerance...
The anti-proliferative effect of these compounds was due to cell death and inducing apoptosis as confirmed by using 4', 6-Diamidino-2-phenylindole (DAPI) fluorescent assay, flow cytometry assay, and finally mitochondrial membrane potential (MMP) staining, Real-time...
Flow cytometry was used to analyze the apoptosis rate. (B) The total percentage of apoptotic cells was depicted with a bar diagram. Data were expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01. 3.8. Docking results To investigate the interaction patten between NT-3 and TNF-α...
Methods: We investigated the presence and functional status of TRAIL and its receptors, DR4, DR5, and DcR2 on tumor as well as tumor-infiltrating lymphocytes (TIL) in primary (n=37), and metastatic gastric carcinoma from malignant ascites (n=37) by a flow cytometry. In addition, ...
Cells were stained with CD4 and CD8 antibodies, and analyzed by flow cytometry. c Total thymocytes prepared in (a) were counted. d To detect apoptosis, total thymocytes prepared in (a) were stained with Annexin V and 7AAD, and analyzed by flow cytometry. e The data indicates the percentage...
Flow cytometry showed that cerebral pericytes express high levels of α5 integrin, and lower levels of α1, α2, and α6 integrins. The pro-inflammatory cytokine tumor necrosis factor (TNF)-α strongly promoted pericyte proliferation and migration, and concomitantly induced a switch in pericyte ...