We also evaluated the expression of two members of the TNFα receptor superfamily, GITR (TNFRSF18) and TNFR2 (TNFRSF1B), both important in T cell biology and expressed by activated T cells [49, 50]. Three days after, we observed a decrease in the percentage of GITR+ cells and MFI ...
Initially, an analysis was done with a fixed ratio of TNF to the receptor (5-fold excess hTNFR1 over hTNF trimer) plus and minus compound UCB-0595 (10-fold molar excess over hTNF trimer). In the sample without compound, as expected, the only form observed was TNF with three receptors...
C57BL/6 mice were provided by the breeding unit of the animal facility of Department of Microbiology, Tumor and Cell Biology, Kar- olinska Institute, Sweden; B6.129S2-Tnfrsf1btm1Mwm/J (Tnfr1 À / À ) mice in C57BL/ 6 background were purchased from the Jackson Laboratory (Bar ...
Representative histograms of ICAM-1 expression with MFI values including fluorescence-minus-one (FMO) control (red) are shown. (E) CD33+CD45lowCD3−CD34+ (CD34+) and CD33+CD45lowCD3−CD34− (CD34−) primary AML blasts from 2 patients were treated with rTNFα. The ICAM-1 MFI ...