We identified changes in growth and proliferation induced by paclitaxel by performing an EdU incorporation assay for three TNBC cell lines (HCC1143, HCC1806 and MDA-MB-468) treated for 72 h with dose escalation of paclitaxel (0.01 nM-81 nM). Higher paclitaxel doses (1 nM-81 nM) induced ...
We identified changes in growth and proliferation induced by paclitaxel by performing an EdU incorporation assay for three TNBC cell lines (HCC1143, HCC1806 and MDA-MB-468) treated for 72 h with dose escalation of paclitaxel (0.01 nM-81 nM). Higher paclitaxel doses (1 nM-81 nM) induced ...
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer that harbors enriched cancer stem cell (CSC) populations in tumors. Conventional chemotherapy is a standard treatment for TNBC, but it spares the CSC populations, which
Subsequently, we narrowed down the list of genes to those that strongly correlated with drug sensitivity of 63 breast cancer cell lines based on Dependency Map Consortium data re-analysis. Furthermore, the list of genes was limited to those presenting specific expression in breast tumor cells as ...
Table 1 List of IC50values obtained for each TNBC cell type, type of drug, and timing response Full size table Fig. 4 Chronic treatment with Pt-compounds induces an increase of NPM1 in HCC70 cells.aRepresentative western blotting shows the NPM1 protein levels trend in HCC70 cells, chronical...
Altei WF, Pachane BC, Souza C, Marques MMC, Selistre-de-Araújo H. New insights into the discovery of drugs for triple-negative breast cancer metastasis. Expert Opin Drug Discov. 2022:1–12. Schmid P, Adams S, Rugo HS, Schneeweiss A, Barrios CH, Iwata H, et al. Atezolizumab and ...
Then, cells were resuspended in Trizol for RNA isolation. The RT2Profiler™ PCR Array for Human Inflammatory Cytokines and Receptors (PAHS-181Z) and Human Cellular Senescence (PAHS-050ZD) were used according to the manufacturer’s protocol (QIAGEN). The detailed list of the genes assessed can...
Fischer TW, Zmijewski MA, Zbytek B, Sweatman TW, Slominski RM, Wortsman J, Slominski A (2006) Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines. Int J Oncol 29:665–672 PubMed CAS Google Scholar Nakamur...
Compared to the controls, gene analysis demonstrated that mutant p53 cell lines decreased the expression of p21 and p15 tumor suppressors upon TGF-β stimulation; however, the gene expression of MMPs and Slug was increased compared to the control, which was correlated with enhanced cellular ...