目前标记通道最多的TMTpro 18-plex标记试剂可以同时比较18例不同样品,但在很多研究中,尤其是临床蛋白质组学研究,需要检测和定量比较的样品数量会多于此数。 这种情况下,通常有两种常见的不同做法以克服标记定量技术中标记通道数量的限制: 一是将多个原始样品等量混合(图3左),以混合样品为单位进行标记,并与其他通道...
一.蛋白鉴定情况 Fig. 2 Area proportional Venn diagrams showing the number of protein identifications and identification overlap of labeled (iTRAQ, TMT) and label-free datasets. 以上结果表明三中检测方式检测出的蛋白大部分相同,而TMT检测蛋白总量最多,而且鉴定蛋白具有较高的蛋白序列覆盖度。 二、前体共选...
Tech Tip: Data acquisition using LC-MS/MS with High Collision Dissociation (HCD) MS/MS of TMT labeled Peptides Product Information User Guide: Immobilized Anti-TMT Resin & TMT Elution Buffer Cookie设置 我们及我们的关联方等通过运用Cookie等技术来为您提供您感兴趣的网站定制内容、识别访问者、保障安全...
Tech Tip: Data acquisition using LC-MS/MS with High Collision Dissociation (HCD) MS/MS of TMT labeled Peptides Relative Quantitation of Protein Digests Using Tandem Mass Tags and Pulsed-Q Dissociation (PQD) Enrichment and Six-Plex Profiling of the DNA Damage Response Pathway ...
Fig. 2 Area proportional Venn diagrams showing the number of protein identifications and identification overlap of labeled (iTRAQ, TMT) and label-free datasets. 以上结果表明三中检测方式检测出的蛋白大部分相同,而TMT检测蛋白总量最多,而且鉴定蛋白具有较高的蛋白序列覆盖度。二、前体共选及TMT/iTRAQ定量评判...
Tandem mass tag (TMT)-based liquid chromatography -tandem mass spectrometry (LC MS/MS) is a proven approach for large-scale multiplexed protein quantification. However, the identification of TMT-labeled peptides is compromised by the labeling during traditional sequence database searches. In this ...
其中,多重同质异素标记的化学交联肽段定量(Quantitation of Multiplexed, Isobaric-Labeled Cross-Linked Peptides, QMIX)方法结合了质谱可切的化学交联剂与同质异素标记试剂,以及新的质谱采集方法。 虽然在Proteome Discoverer 2.2软件的XlinkX 1.0节点中可鉴定TMT标记的化学交联肽段,但无法实现定量。本文采用Proteome ...
Note: TMT-labeled peptide concentration can be measured using Thermo Scientific? Pierce? Quantitative Colorimetric Peptide Assay. The Thermo Scientific? Pierce? Quantitative Fluorescent Peptide Assay cannot be used to measure TMT-labeled peptide concentrations. 8. Optional: Clean-up samples with C18 spin...
In this study, a total of 1464 proteins were quantified in 79 blood samples by depth grading TMT labeled quantitative proteomics, and 1155 proteins were left after the deletion value was filtered. 751 of these proteins were functionally analyzed, and the blood proteome characteristics of non critic...
This study employed TMT-labeled quantitative proteomic technology to identify the differentially expressed proteins (DEPs) in the cervical cancer group when compared with the normal group. This process aided in systematically understanding the protein profiles after the occurrence of cervical cancer. With ...