1. Xu D, ZhuX, Ren J, Huang S, Xiao Z, Jiang H, Tan Y. Quantitative proteomic analysis ofcervical cancer based on TMT-labeled quantitative proteomics. J Proteomics. 2022 Feb 10;252:104453. doi: 10.1016/j.jprot.2021.104453. Epub 2021 Dec 13.PMID: 34915198.2. Brenes A, Hukelmann J,...
Tech Tip: Data acquisition using LC-MS/MS with High Collision Dissociation (HCD) MS/MS of TMT labeled Peptides Relative Quantitation of Protein Digests Using Tandem Mass Tags and Pulsed-Q Dissociation (PQD) Enrichment and Six-Plex Profiling of the DNA Damage Response Pathway ...
However, the motility of yak (Bos grunniens) sperm was low after thawing and the proteomics changes in sperm cryopreservation remain unknown. Therefore, the aim of this study was to explore the differences between fresh sperm and frozen sperm of yak through the proteomic analysis and thus improve...
Furthermore, the cholesterol metabolism pathway, glucagon signaling pathway and glycolysis / gluconeogenesis pathway were noticed that may be the important signal pathways for goat Intramuscular adipocytes differentiation.#By proteomic comparison between goat intramuscular preadipocytes (P_IMA) and intramuscular...
Fig. 2 Area proportional Venn diagrams showing the number of protein identifications and identification overlap of labeled (iTRAQ, TMT) and label-free datasets. 以上结果表明三中检测方式检测出的蛋白大部分相同,而TMT检测蛋白总量最多,而且鉴定蛋白具有较高的蛋白序列覆盖度。
此外，化学致癌物ROS、谷胱甘肽代谢、HIF-1和PPAR等信号通路可能通过影响游离铁过载和GPX4失活来促进铁凋亡。该研究成果中 拜谱生物 为其提供 TMT蛋白组学 的检测与分析，发现的差异蛋白有助于拓展屠宰后牛肉储存过程中铁死亡的生理和分子机制的认识。文章名称：TMT quantitative proteomics analysis reveals molecular ...
蛋白质组学技术正在演变为一种高灵敏度、高通量定量分析方法,可分析细胞、组织或生物体内的全局蛋白质动态。在过去十年间,整个蛋白质组学工作流程取得了重大进展,其中较显著的是串联质谱标签试剂 (TMT)的发展与质谱分析的先进进步,它们提高了高通量(在单个质谱分析中可比...
Analysis of differentially expressed proteins by TMT-based quantitative proteomicsProteomics analysis were performed using protein extractions from parent and spheroid cells from endometrial cancer cell lines. A total of 996,345.0 spectrum were obtained by mass spectrometry analysis. The matched spectrum of...
peptides were desalted by Strata X C18 column (Phenomenex, USA) and freeze-dried in a vacuum. The peptides were dissolved in 0.5 M TEAB and labeled with TMT kit (Thermo, USA) according to the manufacturer’s instructions. Then, the labeled peptide mixtures were combined, desalted and vacu...
Using high-resolution mass spectrometry-based quantitative proteomics of the bovine whey proteins, we identified a total of 1459 and 1358 proteins in biological replicates, out of which 220 and 157 proteins were differentially expressed between normal and infected samples. A total of 82 proteins were...