在ESCMID Global 2024上,浙江大学医学院临床检验诊断学专业的硕士研究生吴钰烨展示了其研究成果—“携带可移动RND型外排泵基因簇tmexCD-toprJ变种的革兰氏阴性细菌的全球涌现”。《感染在线》邀请吴钰烨硕士介绍相关内容。
揭示TmexCD-toprJ基因簇在CRKP中的流行特征及分子传播机制,为控制其进一步播散奠定理论基础.方法:1.对分离自郑州大学某教学三甲医院的991株CRKP分离株的替加环素耐药情况进行分析,筛选替加环素不敏感CRKP株,并检测替加环素不敏感株中TmexCD-toprJ基因簇的携带情况.2.在分析TmexCD-toprJ阳性株耐药谱及临床分布...
引言/目的替加环素是目前已知的治疗多重耐药革兰氏阴性菌感染的"最后一道防线",但新型替加环素耐药基因的出现和传播严重威胁着替加环素的疗效,对全球公共卫生构成严重挑战.最近,在动物源及人源肺炎克雷伯菌中发现的质粒介导的RND型外排泵基因簇tmexCD1-toprJ1,能够降低包括替加环素在内的多种抗菌药物的敏感...
However, the emergence of the tigecycline resistance gene cluster tmexCD-toprJ , which encodes a resistance-nodulation-division efflux pump, has significantly limited its therapeutic effectiveness. This study aims to explore the potential of CRISPR/Cas9-based plasmids to target and cleave tmexCD-to...
本发明公开了一种新型替加环素耐药基因家族tmexCDtoprJ多重PCR检测试剂盒,所述试剂盒由快速PCR混合液,裂解液,超纯水,引物探针混合液,阳性对照品,阴性对照品组成,引物探针混合液包括tmexC引物对,tmexD引物对,toprJ引物对,tmexD2引物对,阳性对照品包括tmexC阳性对照品,tmexD阳性对照品,tmexD2阳性对照品,toprJ...
Novel subtypes and multiple transfer units of tmexCD-toprJ gene clusters were identified in clinical carbapenem-resistant E. cloacae and K. oxytocaplasmidsmobile genetic elementstigecycline resistancedoi:10.1016/j.ijantimicag.2021.106421.28Shijun Sun...
The emergency of two noveltmexCD-toprJvariants necessitates preventive measures to curb their spread and highlights concerns about more emergingtmexCD-toprJvariants. 展开 关键词: Tigecycline resistance Megaplasmid Promoter DOI: 10.1016/j.micres.2025.128051 年份: 2025 ...
RNA-based antibiotic susceptibility testing of tmexCD-toprJ-mediated tigecycline resistance in Klebsiella pneumoniaedoi:10.1186/s44280-023-00025-9Yu, FeiyuZhang, HaijieZhu, ShuyaoWang, ZhiqiangLiu, YuanOne Health Advances
tmexCD-toprJbenzydamineGram-negative bacteriaRecently, a novel efflux pump gene cluster called tmexCD1-toprJ1 and its variants have been identified, which undermine the antibacterial activity of tigecycline, one of the last remaining options effective against multidrug-resistant (MDR) Gram-negative ...