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Herein, we found that miR-574-5p, which was upregulated in both human and mouse lupus, acted as a potent ligand for mouse mTlr7 and human hTLR8 but not hTLR7. Aberrant activation of miR-574-5p-hTLR8/mTlr7 signaling was found to be associated with significant dysregulation in immune an...
miR-574-5p is significantly up-regulated in the serum samples from human SLE patients and the serum and other tissues of lupus-prone B6.Faslprmice. miR-574-5p levels were determined by qPCR as described in the Extended Materials and Methods in the Supplementary Information. NS, not significant...
After various treatments, cells were fixed with 2% formaldehyde and stained with Hoechst 33342 (Invitrogen), The coverslips were mounted on slides and then examined using a confocal laser microscope (Nikon C1 plus, Bioimaging Shared Resource of the Kimmel Cancer Center (NCI 5 P30 CA-56036)). ...