The effects of deficiency of Factor XII or its enzymatic substrate, prekallikrein, were examined in the imiquimod‐induced mouse model of psoriasis. Skin microcirculation was assessed using intravital confocal microscopy and laser Doppler flowmeter. A novel antibody blocking Factor XII activation was eval...
For quantification of microglia density, whole-section montages were created using a confocal laser scanning microscope (TCS SP8, Leica, Wetzler, Germany) with Leica Application Suite software and a ×10 objective. All images were acquired under identical parameters and exported to NIH ImageJ for ...
TLR7 promotes pathogenesis of systemic lupus erythematosus. However, whether RNA structural changes affect nucleic acids-sensing TLRs signaling and impact disease progression is unclear. Here by iCLIP-seq we identify a TLR7-binding long non-coding RNA, Lnc-Atg16l1, and find that ...
we studied the effect of formulation on potency of a 1,2‑di‑(9Z‑octadecenoyl)‑sn‑glycero‑3‑phosphoethanolamine (DOPE) conjugated TLR7 agonist (DOPE-TLR7a) alongside assessing physical form using Dynamic Light Scattering (DLS), Nanosight Particle Tracking (NTA) analysis and Small...
Finally, from the perspective of understanding the solute carrier family and its biology, our findings further highlight the fact that SLCs can have, behind their canonical role as transporters, additional, still underappreciated functions by acting as transceptor (transporter-receptor) and signaling com...
the tumor microenvironment were dependent on stromal or neoplastic cell TLR7. Finally, to explore therapeutic viability of R848 in human populations, we compared stromal and epithelial expression ofTlr7and related transcripts by RNA-sequencing (RNA-seq) in laser-capture microdissected human lesions ...
TLR3 is shown in green, CD25 and CD25-EG∼ID are shown in red (pseudocolor), and CTXb is shown in light blue (pseudocolor). We further examined whether the linker region of TLR3 targets CD25 to an intracellular compartment where TLR3 is located. Both TLR3YFP and CD25CFP or CD25-...