The use of normal as well as reversed-phase systems fails to resolve highly polar compounds that are strongly adsorbed on polar stationary phases and poorly retained on alkyl-bonded stationary phases usually used in HPLC. Because of these facts, separation has been performed in hydrophilic ...
英文版TLC技术介绍
between the silica gel plate and the development solvent for the spotted material. The very polar silica gel tries to hold the spot in its original place and the solvent tries to move the spot along with it as it travels up the plate. The outcome depends upon a balance among three polar...
their properties indicated by the solvation parameter model. This suggests co-mingling of solute and solvent properties among the main parameters of the competition model leading to additional uncertainty in model predictions for varied compounds. The reduction in the contribution of site-specific and st...
Hajnos, M. Waksmundzka-Hajnos, Application of hydrophilic interaction TLC systems for separation of highly polar glycosidic compounds from the flowers of selected Verbascum species, Planar Chromatogr. -Mod. TLC 24 (2011) 295-300.L. Cies´la, M. Hajnos, and M. Waksmundzka-Hajnos, "...
– silica gel) and a liquid phase (or mobile phase) containing the solute (mixture - extract with pigments in this case). The mobile phase can be chosen depending on the purpose, for example for separating polar (hydrophilic, or water soluble) molecules in the mixture one would use polar ...