浅述TIR1介导的生长素信号通路 Aux/IAA-TIR1-ARF 信号通路在植物生长、发育和环境响应中扮演着关键角色。TIR1(Transport Inhibitor Response 1)是这一信号通路的核心蛋白,也被称为AUX/IAA转录因子降解蛋白。TIR1位于细胞核中,充当生长素受体的角色,与生长素分子IAA结合,启动生长素信号传导。 在没有或生长素浓度很...
The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1 , was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The ...
伴随着拟南芥(Arabidopsis thaliana)作为模式植物的广泛应用, Estelle和Leyser 两个团队在 2005 年同时独立发现 F-box 蛋白 TIR1 (Transport Inhibitor Response 1)是生长素的受体, 使生长素信号转导通路的研究取得飞速发展.后续研 究表明, 生长素受体TIR1及其同源受体蛋白AFBs (Auxin Signaling F-box Proteins)能够...
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胚性愈伤组织生长素受体基因TIR1(Transport inhibitor response 1,即DL-TIR1)和生长素结合蛋白基因ABP1(... 李惠华,赖钟雄,苏明华,... - 《园艺学报》 被引量: 0发表: 2012年 茶树生长素受体基因CsTIR1的克隆与表达分析 Cs TIR1在茶树的根、茎、叶和花中具有组织表达特异性;其表达受IAA诱导,3种不同浓度...
摘要: 采用RT-PCR结合RACE法,分离克隆龙眼(Dimocarpus longan Lour.)胚性愈伤组织生长素受体基因TIR1(Transport inhibitor response 1,即DL-TIR1)和生长素结合蛋白基因ABP1(Auxin biding protein 1,即DL-ABP1),运用生物信息学方法对序列进行分析,并通过实时荧光定量P... 查看全部>>关键词:...
TIR1(transportinhibitorresponse1)基因是最早对拟南芥生长素运输抑制剂萘基酞氨酸(naphthylphtha2lamicacid,NPA)遗传检测时鉴别出来的,因此得名“生长素运输抑制剂反应1”,即TIR1。但其后发现TIR1并不参与生长素的运输,而是与生长素作用有关。对该基因进行克隆表明,TIR1是一个F2box蛋白,与泛素化蛋白降解途径密切相关...
摘要 以野生型拟南芥(Arabidopsis Columbia)基因组DNA为模板,通过PCR扩增得到拟南芥生长素受体基因TIR1启动子2008片段,将该片断克隆到PGM-T载体上。序列分析表明,该启动子大小为2008bp,R...展开更多 TIR1 (transport inhibitor response l ) is an auxin receptor, TIR1 promotor was amplified from Arabidopsis ...
本文主要进行了水稻生长素结合蛋白(auxin-binding protein, ABP1)和生长素受体(transport inhibitor response, TIR1)的原核表达与纯化研究,获得了以下研究 结果: 1.以超级杂交稻株1S为材料,根据GeneBank中公布的ABP1及TIR1 cDNA序列设计引物,通过RT-PCR的方法克隆出生长素结合蛋白ABP1基因和生长素受体蛋白TIR1基因,...
Auxin is a pivotal plant hormone that controls many aspects of plant growth and development. Perceived by a small family of F-box proteins including transport inhibitor response 1 (TIR1), auxin regulates gene expression by promoting SCF ubiquitin-ligase-