In this study, a timsTOF Pro instrument (Bruker Daltonics) was used, with the eluted ions further analyzed via quadrupole time-of-flight mass spectrometry (Figure 2). The spectra generated during the TIMS elution cycle is termed a TIMS scan. The two dime...
Parallel Accumulation and Serial Fragmention (PASEF) [2] on the timsTOF Pro platform makes efficient usage of the ion beam and with intelligent precursor placement within a TIMS cycle achieves rapid sequencing speed. In addition, the ions get focused in space and time within the TIMS cell, ...