rna-seq snakemake slam-seq tuc-seq timelapse-seq nr-seq Updated Dec 10, 2024 Python Improve this page Add a description, image, and links to the timelapse-seq topic page so that developers can more easily learn about it. Curate this topic Add this topic to your repo To associate...
Here we report a protocol for TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. The steps of the protocol are (1) treating samples with 4-thiouridine ...
Jeremy A Schofield, Erin E Duffy, Lea Kiefer, Meaghan C Sullivan, and Matthew D Simon. Timelapse-seq: adding a temporal dimension to rna sequencing through nucleoside recoding. Nature methods, 15:221-225, March 2018. ISSN 1548-7105. doi: 10.1038/nmeth.4582....
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that ...