Here we report a protocol for TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. The steps of the protocol are (1) treating samples with 4-thiouridine ...
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that ...
I import a time lapse seq into light room, edit them here then export them to JPG. (filename + seq number ) Then once importing into after effects A frame from the start of the time lapse constantly comes at random times throughout the whole time lapse? Any suggestions? Views...
I like doing AEB seqyences even, to make HDR of some of it - but then only use normal exposure ones for time-lapse. Unless camera allow to make time-lapse video in camera. Excuse me if kicking in open doors and you know this already. Votes 1 Upvote Translate Translate Report Report...
execute("seq.every(a, seq.and(seq.ge(0), seq.lt(10)))", env); // Lambda 求和 AviatorEvaluator.execute("reduce(a, lambda(x,y) -> x + y end, 0)", env); // 导入 String 类实例方法作为自定义函数 AviatorEvaluator.addInstanceFunctions("s", String.class); AviatorEvaluator.execute(...
链接：https://pan.baidu.com/s/1eSeQwvw密码：819r 或者 https://aeziyuan.ctfile.com/fs/4861363...
TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in traditional RNA-seq.doi:10.1038/nmeth.4582Schofield, Jeremy ADuffy, Erin EKiefer, Lea