应用领域:YFP 中枢系统 参考文献 Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ...
Thy1-YFP transgenic miceWallerian degenerationaxonclearoptical clearingunobstructed brain imaging cocktails and computational analysisOBJECTIVE :Wallerian degeneration is a pathological process closely related to peripheral nerve regeneration following injury, and includes the disintegration and phagocytosis of ...
mice created by back-crossing the original B6.Cg-Tg(Thy1- YFP)16Jrs/J mouse strain to BALB/c genetic background. Two tumour cell lines were tested, the 4T1 mammary carcinoma and the MC26 colon carcinoma, respectively. In the case of s.c. tumour injections in the ear pinnae, in the...
A simple head-mountable LED device for chronic stimulation of optogenetic molecules in freely moving mice The device is easily mountable on the head of a mouse with a high-polymer block. Using the Thy1–ChR2–YFP transgenic mice, we demonstrate that the... Y Iwai,S Honda,H Ozeki,... -...
Cyan fluorescent protein (CFP) expressing cells in the retina of Thy1-CFP transgenic mice before and after optic nerve injury We investigated the specificity of cyan fluorescent protein (CFP) expression in retinal ganglion cells (RGCs) of the transgenic Thy1-CFP (B6.Cg-Tg(Thy1-CFP......
Additional proof that Thy1 marks a distinct non-T-cell population of epidermal cells was obtained by performing immunofluorescence staining on dorsal skins from adult telogenic (P56) athymic (Foxn1nu) nude mice. Our data clearly demonstrate the presence of Thy1+basal keratinocytes co-labeled with...
but it was difficult to obtain image information of the entire sample.At the same time,the image information obtained by fluorescence imaging and HE staining was partially lost.Conclusion:The combining of CUBIC and Thy1-YFP transgenic mice allowed for a clear and comprehensive observation of ...
Yan Y, Sun HH, Mackinnon SE, Johnson PJ (2011) Evaluation of peripheral nerve regeneration via in vivo serial transcutaneous imaging using transgenic Thy1-YFP mice. Exp Neurol 232:7-14.Evaluation of peripheral nerve regeneration via in vivo serial transcutaneous imaging using transgenic Thy1-YFP ...
In conclusion, stem cells originating from Thy1 YFP-16 mice represent an outstanding tool to monitor neurogenesis enabling morphological analyses of new neurons and their projections, in particular after transplantation in the brain.doi:10.1016/j.neulet.2016.10.001Ali, Ivan...
Methods :Retinas from Thy1-YFP-H mice were processed for immunohistochemistry (ChAT) and fluorescence imaging (Z-stacks, LeicaTCSSP8 confocal microscope). Dendritic arbours were traced with the filaments Tree Autopath Algorithm (plugin, Imaris), and Sholl analysis (plugin, Imaris) performed on each...