Three-dimensional (3D) cell cultures represent a new and robust platform for studying complex biological processes and are a promising alternative in regenerative and translational medicine. Expert opinion: Three-dimensional cell cultures are obtained by combining several types of cells in integrated ...
After Ano5 gene knock-down with shRNA in MC3T3-E1 osteoblast precursors we saw elevated expression of osteoblast-related genes such as Col1a1, osteocalcin, osterix and Runx2 as well as increased mineral nodule formation in differentiating cells. Our data suggest that ANO5 plays a role in ...
Cleft Alveolus Reconstruction Using a Three-dimensional Printed Bioresorbable Scaffold with Human Bone Marrow CellsIn this study, we adapted in-house 3D printer using extrusion-based dispensing method to print a defect-customized scaffold and successfully prepared the scaffold for the alveolar bone defect...
namely SARS-CoV, MERS-CoV and SARS-CoV-2. The economic burden and health threats caused by these coronaviruses are extremely dreadful and getting more serious as the increasing number of global infections and attributed deaths of SARS-CoV-2 and MERS-CoV. Unfortunately, specific medical countermeas...
Interestingly, a 3D microtissue system composed of cardiomyocytes, cardiac endothelial cells, and cardiac fibroblasts, the three major cell types of the heart, derived entirely from human iPSCs, can represent an excellent resource to study the differentiation of human heart cells in the development ...
This structural randomness resulting from these techniques has demonstrated implications for biological function, causing heterogeneity in the distribution of cells in vitro and non-uniform tissue ingrowth in vivo [13,16,17,18]. Three-dimensional printing (3DP) technology in BTE (workflow schematic ...
alveolus by reducing surface tension at the air-liquid interface. The Immortalized Mouse Lung Epithelial Cells (MLE-15) is a cell line derived from transgenic FVB/N mice harbouring SV40 TAg under the control of the human SP-C promoter. Similar to alveolar type II cells, MLE-15 is ...
The results of this study indicate that the mechanism of induction and release of chemokines is different when stimulated either by particles or polysaccharides. In addition, the effects of LPS on these cells were more extensive than those observed with other stimulants alone. Qt induces primarily ...