The first problem, and the focus of the first part of this work, is to understand the behavior of proteins in the complex milieu of a living cell. We have developed 3D In-Cell protein NMR and demonstrated its use to assign the backbone resonances of a protein in living E. coli cells....
Single-molecule localization microscopy is a powerful tool for visualizing subcellular structures, interactions and protein functions in biological research. However, inhomogeneous refractive indices inside cells and tissues distort the fluorescent signal emitted from single-molecule probes, which rapidly degrade...
DUF1996 Proteins Localize in Vacuoles and Function in Fungal Responses to Multiple Stresses and Metal Ions Sen-Miao Tong,Ying Chen, Sheng-Hua Ying & Ming-Guang Feng Many annotated fungal genomes harbour high proportions of hypothetical proteins with or without domains of unknown function (...
Briefly describe five functions of membrane proteins. Why does a eukaryotic cell need so many compartments(organelles)? What is the function of the nucleus? How do genes in the nucleus control the functions of other parts of the cell? What is ...
We suggest that AmMYBML1 is regulated by DIV in association with the B-function proteins DEFICIENS and GLOBOSA, and, consequently, controls specification of particular cells within the ventral petal which adapt the corolla to specialised functions in pollination. 展开 ...
The interpretation of morphogen gradients is a pivotal concept in developmental biology, and several mechanisms have been proposed to explain how gene regulatory networks (GRNs) achieve concentration-dependent responses. However, the number of different mechanisms that may exist for cells to interpret mo...
The glycosaminoglycan hyaluronan (HA) and HA-binding proteins (HABPs) serve important structural and regulatory functions during development and in maintaining adult tissue homeostasis. Here we have identified and partially characterized the sequence and expression pattern of three putative novel HABPs. DN...
Epigenetic modifications and spatial proximities of nucleic acids and proteins play important roles in regulating physiological processes and disease progression. Currently available cell imaging methods, such as fluorescence in situ hybridization (FISH)
This classic Hi-C study describes A/B compartment characteristics and dynamics in differentiating mouse B cells, revealing different types of DNA-binding proteins associated with chromatin interactions at different length scales. Article CAS PubMed PubMed Central Google Scholar Hill, L. et al. Wapl...
It is thus hypothesised that it is the ratio of short:long Homer isoforms that is of functional importance, particularly in maintaining calcium homeostasis (Kaja et al., 2013), and that the functions of long and short Homer isoforms vary according to brain region and cell-type (Ango et al....