TLR激动剂抑制IL-27诱导的THP-1细胞HLA-DR的表达 目的 研究白介素27(IL-27)对THP-1单核细胞系Ⅱ类转录活化子(class Ⅱ transactivator,CⅡTA)和Ⅱ类主要组织相容性复合物(major histocompatibility complex class Ⅱ,MHC Ⅱ)分子的表达的影响及Toll样受体(Toll-like receptor,TLR)激动剂LPS和Pam3CSK4的干预作...
同时,HLA-DR的表达也上调,表明细胞具备更强的抗原提呈能力。这些结果表明PMA处理可以诱导THP1细胞分化为具有更强免疫功能的巨噬细胞。 本文通过流式细胞术分析发现,PMA处理可以诱导THP1细胞分化为具有明显巨噬细胞表型特征的细胞。这些结果表明,PMA可能成为一种有效的诱导分化剂,用于研究和治疗巨噬细胞相关疾病。然而,...
Immunology: CD3 -, CD4 +, CD13 +, CD14 (+), CD15 +, CD19 -, CD33 (+), CD34 -, CD68 +, HLA-DR + Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH, NP Cytogenetics: human near-tetraploid karyotype ...
(+), CD34 -, CD68 +, HLA-DR + Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH, NP Cytogenetics: human near-tetraploid karyotype - 94(88-96)4nXY/XXY, -Y, +1, +3, +6, +6, -8, -13, -19, -...
It can therefore be presumed that AP suppresses HLA-DR expression via the ubiquitin鈥損roteasome pathway. Furthermore, the up-regulation of IL-12 and TNF-伪 was found in the procyanidin trimers-treated cells in the presence of OVA. These results suggest that apple polyphenols would be an ...
分别检测细胞上CD90、CD105、CD73、CD79a、CD34、CD11b、CD19、CD14、CD45和HLA-DR标记性分子的表达情况,分别检测细胞向脂肪、成骨和软骨分化的能力。结果显示我们分离出的细胞90%以上表达CD90、CD105和CD73,而CD79a、CD34、CD11b、CD19、CD14、CD45和HLA-DR几乎不表达 (图2A),并且该细胞可以向脂肪、成骨...
方法以10 ng/mL佛波酯诱导THP-1人单核细胞,建立分化的巨噬细胞模型,分别以100 U/mL IFN-γ和100 ng/mL巨噬细胞集落刺激因子(M-CSF)刺激分化的THP-1细胞,获得M1型和M2型巨噬细胞,利用实时定量PCR(qRT-PCR)、流式细胞术和Western blot法检测巨噬细胞HLA-DR、CC型趋化因子受体7(CCR7)、IL-12p35、IL-10...
THP-1 is an acute monocytic leukemia cell line which acquires phenotypic and functional monocytoid-like features following incubation with mezerein. The current study concerned the modulation of these features by rIFNγ. rIFNγ induces the time-dependent enhancement of HLA-DR expression in the pres...
THP-1细胞表面CD83、CD11c和HLA-DR的表达均较高,表达量分别达到(54.75±7.84)%、(76.15±9.12)%和(94.25±2.89)%,CD1a分子的表达呈阴性。THP-1-M1和THP-1-M2细胞则高表达这几个分子。与之相反,THP-1-Mφ细胞CD1a的表达仍为阴性,CD83分子的表达明显降低(P=0.0498),CD11c和HLA-DR的表达更是显著下降,P值...
Circulating monocyte subset distribution in preeclampsia is altered in comparison to healthy pregnancy: an increased number of intermediate monocytes (CD14hi/CD16+/HLA-DR+) together with a decreased classical monocyte subset (CD14hi/CD16-/CCR2hi) and increased non-classical monocyte subset(CD14low/...