LAMBRECHTS N ET AL: "THP-1 monocytes but not macrophages as a potential alternative for CD34 dendritic cells to identify chemical skin sensitizers", TOXICOLOGY AND APPLIED PHARMACOLOGY, ACADEMIC PRESS, US, vol. 236, no. 2, 15 April 2009 (2009-04-15), pages 221-230, XP026029805, ISSN: ...
目的:利用不同的人巨噬细胞株,观察其在卡介苗(BCG)诱导下产生一氧化氮(NO)的可能性,探讨人体巨噬细胞在吞噬、杀灭结核分支杆菌过程中NO的作用.方法:培养THP-1及U-937巨噬细胞,用抗酸染色方法,观察THP-1及U-937巨噬细胞对BCG的吞噬率;设不同细胞数组,添加不同剂量BCG,用Griess法检测上清液中NO的含量.结果:U...
结核杆菌H37Ra感染RAW264.7巨噬细胞株24 h后收集上清液,用Griess法检测一氧化氮(NO),化学法检测过氧化氢(H2O2)的产生,逆转录~聚合酶链反应(RT~PCR)法检测感染后巨噬细胞IL~12和TNF~αmRNA的表达,ELISA法检测细胞因子IL~12和TNF~α的含量。结果:结核杆菌H37Ra感染巨噬细胞后产生的NO和H2O2水...
Integrins have no intrinsic enzyme activity; instead, they transduce signals through conformational changes and protein–protein interactions, leading to the tight adhesion of monocytes to the vascular endothelium and the induction of active, cytoskeleton-driven monocyte migration [7]. Integrins can ...
Free Radic Res 31:367-379.J. L. Wood and A. Graham, "Reduction of transition metals by human (THP-1) monocytes is enhanced by activators of protein kinase C," Free Radical Research, vol. 31, no. 5, pp. 367- 379, 1999.
No Tumorigenic - Effects - Comments The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin.Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Culture Method Doubling Time 26...
NO.1 收货时细胞出现伪足,或是碎片较多怎么办? 受到运输影响,悬浮细胞会短暂性出现形态改变的现象,如伪足等。通过传代培养,1周左右可以恢复正常。一些细胞在运输途中死亡而产生碎片,通过传代离心可以去除(见上文传代篇)。 NO.2 培养过程中细胞发生贴壁怎么办?
NO.1收货时细胞出现伪足,或是碎片较多怎么办? 受到运输影响,悬浮细胞会短暂性出现形态改变的现象,如伪足等。通过传代培养,1周左右可以恢复正常。一些细胞在运输途中死亡而产生碎片,通过传代离心可以去除(见上文传代篇)。 NO.2培养过程中细胞发生贴壁怎么办?
目的:了解问号钩端螺旋体(简称钩体)对小鼠和人单核-巨噬细胞株诱导型一氧化氮合酶(iNOS)表达及 一氧化氮(NO)水平的影响,了解不同宿主巨噬细胞对问号钩体的杀菌机制.方法采用问号钩体56601株感染J774A.1及人单核细胞株THP-1建立 钩体细胞感染模型,采用re-al-time RT-PCR法和流式细胞术分别测定细胞iNOS mRNA...
However, there was no significant signal transduction in either the mCRP or pCRP treated group through these pathways when compared to control. Whilst this does not demonstrate the signaling mechanism of either CRP group, it is clear that it differs substantially from that induced by LPS. This ...