We hypothesized that LPS stimulation of the human monocyte cell line THP-1 occurs via MAPK activation, and that LPS desensitization, induced by pre-exposure to LPS, is associated with altered signaling through the MAPK cascade. Involvement of a specific MAPK, ERK, in LPS-stimulated TxB2 ...
目的 观察不同浓度脂多糖(LPS)刺激THP-1细胞(human acute monocytic leukemia cell line)不同时间对可溶性CD14(soluble CD14,sCD14)和膜CD14(Membrane CD14,mCD14)表达的影响及sCD14,mCD14表达之间的关系.方法 不同浓度LPS(0,0.1,1.0,10,50ng/ml)刺激经佛波酯(PMA)诱导的THP-1细胞不同时间(0.5,1,3,5,8...
Kim SJ, Park JH et al (2011) Melittin inhibits atherosclerosis in LPS/high-fat treated mice through atheroprotective actions. J Atheroscler Thromb 18(12):1117–1126 Article CAS PubMed Google Scholar Lee JS, Park SY et al (2010) Grifola frondosa water extract alleviates intestinal inflammation ...
Stimulation with LPS failed to affect the expression of EP 1 and EP 3 receptor proteins , 华中科技大学硕士学位论文 4 but upregulated the expression of EP 2 and EP 4 receptor proteins in human monocyte line THP-1. The results suggest that proinflammatory stimuli probably regulate the initiation...
inTHP一1cellsunderstimulationofLPS ,whileshowednoinhibit0rye ct onTREM一1mRNA.ThesecretionlevelsofinflammatoryfactorsweresignificantlylowerinthesupernatantsofTHP一1ce11sinLPS+ TREM一1/FCandLPS+AzithromycingroupsthaninLPSgroup(P<0.01),whilewerelowerinLPS+TREM一1/FCgmupthanin LPS+ Azithromycingroup. ...
Inflammatory marker genes (TLR-4, TNF-α, NF-κB and its subunit genes p52, p50, RelB, c-Real, p65) were expressed in U937 cells in a wide range of LPS stimulations of 0.3 ng/ml–2.5 μg/ml [12–14]. Sharif et al. [15] described that U937 monocytes responded to 2 μg/ml ...
Differentiated THP-1 cells were preconditioned with subthreshold concentration (0.03 microg/ml HSP-70) for 18 h, followed by LPS stimulation (1 microg/ml) for 4 h. Preconditioning with HSP-70 decreased subsequent LPS-mediated NF-kappaB-dependent promoter activity and was accompanied by significant...
(P<0.05).After restimulations for another 24 h,TNF-α production was decreased significantly compared with that secreted by the cells stimulated with the same LPS only once (P < 0.05),while the level of IL-10 was increased significantly (P < 0.05).Conclusion:Repeated LPS stimulations ...
Furthermore, cell experiments revealed that LPS stimulation of either THP-1 cells pre-incubated with post-exercise plasma or peripheral blood mononuclear ... SM Hurst,KA Lyall,RD Hurst,... - 《European Journal of Applied Physiology》 被引量: 10发表: 2009年 Preparation and characterisation of am...
究发现 ,芍药苷可抑制脂多糖 (LPS)诱 导的小 鼠单 核巨噬细胞株 (RAw 264.7) 高迁移 率族蛋 白 B1 (HMGB1) 的核转位 ,从而对 LPS 诱导 的肝损害起 保护作用 。但关 于芍药苷是否可 以抑制 BLP 诱导 T HP一1 细胞产生炎症反应 的研究 尚没有报道 。本 ...