THP1-Dual™ KO-IRF5 cells were generated from the human THP1-Dual™ monocyte cell line by stable knockout of the interferon regulatory factor 5 (IRF5) gene. These cells feature two inducible reporters allowing the simultaneous study of NF-kB and IRF
THP1-Dual™ KO-RIG-I cells express two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively. In addition, these cells feature a stable knockout of ...
英 文名IRF9 gene knockout cell(monoclonal) 规格1T 品 牌圣尔生物share-bio 一键复制产品信息 产品编号名称包装品牌目录价促销价货期数量购物车 SB-KO24403IRF9基因敲除THP-1细胞(单克隆)IRF9 gene knockout cell(monoclonal)1T圣尔生物share-bio¥9000.00 ...
目的 研究白介素27(IL-27)对THP-1单核细胞系Ⅱ类转录活化子(class Ⅱ transactivator,CⅡTA)和Ⅱ类主要组织相容性复合物(major histocompatibility complex class Ⅱ,MHC Ⅱ)分子的表达的影响及Toll样受体(Toll-like receptor,TLR)激动剂LPS和Pam3CSK4的干预作用.方法 RT-PCR检测CⅡTA Ⅰ、Ⅲ和Ⅳ以及人白细胞...
IRF Reporter (Luc) - THP-1 Cell line (cGAS-Sting Signaling Pathway) 注册证号: / 规格: 2管 The Interferon Regulatory Factor (IRF) reporter (Luc)-THP-1 cell line is designed to study the activation and signaling of Cytosolic DNA Sensors (CDS) in human monocytic cell line THP-1....
THP-1 cell line (ATCC® TIB-202™) or THP-1 Dual (Invivogen NF-κB-SEAP IRF-Luc Reporter Monocytes) 2. PMA (Phorbol 12-myristate 13-acetate) (Millipore Sigma P1585) 3. Culture Media: a. RPMI 1640 (Gibco #11875093) b. 10% Fetal Bovine Serum (Corning) c. 1% l-Glutamine (Gi...
(interferon regulatory factor-1,IRF-1)mRNA的表达.半定量PCR检测HLA-DRA,DRB,DPA,DPB,DQA,DQB,IRF-1,CⅡTA mRNA的表达.流式细胞术检测细胞表面HLA-DR的表达.结果IL-27刺激24h后可分别上调THP-1细胞CⅡTAⅢ,CⅡTAⅣ和IRF-1mRNA表达水平.IL-27刺激24h和48h可升高MHCⅡ类分子mRNA的表达,并能诱导HLA-DR...
THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs. As a result, THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of SEAP, and the IRF pathway, by assessing th...
THP1-Blue™ ISG-KD-STING cells allow the monitoring of IRF activation by determining the activity of SEAP. The levels of IRF-induced SEAP in the cell culture supernatant are readily assessed with QUANTI-Blue™, a SEAP detection reagent. THP1-Blue™ ISG-KD-STING cells are resistant to ...
I 型干扰素还可诱导 ISGF3 复合物的形成,该复合物由 STAT1、STAT2 和 IRF9 组成。ISGF3 复合物与 ISRE(干扰素刺激反应元件)结合,进一步诱导启动子内含有 ISRE 的干扰素刺激基因的转录。 IFNγ 与 IFNγ 受体结合导致下游 STAT1 在 Tyr701 位点发生酪氨酸磷酸化。产生磷酸化的 STAT1 同型二聚体转位至细胞核...