、787737分类号——UDC密级——编号南华大学硕士学位论文B类I型清道夫受体在小型猪7A.HepG2、THP-I细胞中表达的变化研究生姓名:指导教师姓名**..
Effects of an 11-nm DMSA-coated iron nanoparticle on the gene expression profile of two human cell lines,THP-1 and Hep G2. Ling Z,Xin W,Zou J,et al. J Nanobiotechnol . 2015Zhang L, Wang X, Zou J, Liu Y, Wang J. Effects of an 11-nm DMSA- coated iron nanoparticle on the ...
目的:通过RNAi技术探讨LITAF在软脂酸引起的肝细胞胰岛素抵抗中的作用,寻找改善肝脏胰岛素抵抗的新的靶位点;方法:设计合成LITAF的特异有效的siRNA.将肝细胞和THP-1源巨噬细胞共培养,分为组1.scramble组,该组中的THP-1源巨噬细胞转染scramble siRNA;组2.LITAFsiRNA组,该组中THP-1源巨噬细胞转染LITAFF siRNA及组3....
而最后一步则是HDL中的胆固醇酯经选择性吸收而转运到肝脏的过程.作为HDL的唯一受体,SR-BI在RCT中起着十分重要的作用.本研究拟在动脉粥样硬化小型猪模型上观察肝脏和血管SR-BI表达的变化,然后采用ox-LDL,LDL及HDL分别处理肝HepG2细胞和肝外THP-1巨噬细胞,观察细胞水平上SR-BI的表达及细胞内胆固醇转运的变化,为...
Human SCD1 coding sequence was amplified by PCR using human HepG2 hepatocyte-derived cDNA as a template with primers tailed with BamHI and EcoRI restriction sites. The PCR product was sub-cloned to lentiviral expression plasmid pCDH-CMV-MCS-EF1-copGFP (System Biosciences; Mountain View, CA)...
E x p r e s s i o no f T o x o p l a s m a g o n d i i R O P １６p r o t e i n i nT H P Ｇ１h u m a n l e u k e m i a c e l l s a n d i t s e f f e c t s o n p r o l i f e r a t i o na n da p o p t o s ...
目的:观察ATP结合盒转运体A1(ABCA1)和ABCG1在肺炎衣原体(C.pn)诱导 THP-1源性泡沫细胞形成中的作用,以初步探讨C.pn诱导泡沫细胞形成的分子机制.方法:C.pn在Hep-2细胞内增殖,将不同浓度的 C.pn(1×10^5-1×10^6 IFU)分别感染THP-1单核细胞源性巨噬细胞0～72小时,运用油红O染色观察细胞浆内脂滴的变...
The expression levels of endogenous apoptotic pathway key proteins Bcl-2, Bax, cytochrome C, Apaf-1, cleaved-Caspase9 were detected using Western blotting. (B–F) Endogenous apoptotic pathway key results of grey-scale analysis of protein expression. (G) THP-1 cells were transfected with siRNA-...
运用双向荧光差异凝胶电泳分析VLDL致THP-1来源泡沫细胞的形成
08 February 2016 THP-1 CellsThrough MAPK and AP-1 Pathways Monica Currò1, Roberto Risitano1, Nadia Ferlazzo2, Santa Cirmi2, Chiara Gangemi1, Daniela Caccamo1, Riccardo Ientile1 & Michele Navarra2 Flavonoids have been shown to be effective in protecting against age-related ...