Day 1 1. Add 180 µl of THP1-Dual™ cell suspension per well of a 96-well plate (~100,000 cells/well). 2. Treat cells with 20 µl of PMA (final concentration 20-50 ng/ml) for 3 hours at 37 °C, 5% CO2. 3. Wash cells gently with pre-warmed PBS and ...
THP1-Dual™ hTLR7 KO-TLR8 Cells Human TLR8 Knockout and TLR7 Overexpressing - THP-1 Reporter Monocytes Add to favorite 3-7 x 10e6 cells thpd-htlr7-ko8 ￥29,408 Please contact our distributor THP1-Dual™ hTLR7 KO-TLR8 vial Additional cell vial Add to favorite 3-7 x...
产品名称: THP1-Dual? Cells .0 订购此产品 供应商: Invivogen 规格: 3-7 x 10e6 目录价: 16507.5 库存状态: 三周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: THP1-Dual™ Cells THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration...
THP-1 cell line is a suitable in vitro cell model to study modulation of monocyte and macrophage functions. • The use of THP-1 cell line can help supporting the eventual in vivo proven bioactivities of food compounds and pharmacological activities of drugs. • THP-1 cell line can hint ...
上海玉博生物科技有限公司做为invivogen代理商之产品thpd-kostg-THP1-Dual KO-STING Cells 3-7 x 10e6 cells的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。
The proliferative ability of cells in each group was detected by CCK-8 assay, and the cell cycle was detected by flow cytometry. TargetScan software was applied to predict the miR-126-3p target gene as RGS3, which was verified by dual luciferase reporter gene assay. The protein expression ...
上海玉博生物科技有限公司做为invivogen代理商之产品thpd-r232-THP1-Dual™ KI-hSTING-R232 Cells 3-7 x 10e6 cells的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。
THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells. This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various...
Cell preparation 1. Centrifuge at 150 x g (RCF) for 10 mins or 300 x g (RCF) for 5 mins. 2. Remove supernatant and resuspend THP1-Dual™ hTLR9 cells at 5 x 105 cells/ml in freshly prepared, pre-warmed test medium. Detection of NF-κB induction 1. Add 20 µl of...
KO-RIG-I dual reporter monocytes for RLR pathway studies Reporter systems in THP1-Dual™-derived cells InvivoGen offers a new series ofTHP1-Dual™cell lines, derived from the human THP-1 monocytic cell line, and specifically designed for assessing the role ofRIG-I,MDA5, andMAVSin the...