Day 1 1. Add 180 µl of THP1-Dual™ cell suspension per well of a 96-well plate (~100,000 cells/well). 2. Treat cells with 20 µl of PMA (final concentration 20-50 ng/ml) for 3 hours at 37 °C, 5% CO2. 3. Wash cells gently with pre-warmed PBS and ...
Cell preparation 1. Centrifuge at 150 x g (RCF) for 10 mins or 300 x g (RCF) for 5 mins. 2. Remove supernatant and resuspend THP1-Dual™ hTLR9 cells at 5 x 105 cells/ml in freshly prepared, pre-warmed test medium. Detection of NF-κB induction 1. Add 20 µl of...
3.2.1 THP1-Dual cell reporter assay 在细胞学平台拓展和方法研究方面,爱思益普细胞学团队通过“reporter assay”检测方法验证不同反应(NF-κB和ISRE)条件下,经2’3’-cGAMP处理后,THP1-Dual 荧光素酶报告系统荧光值变化(图3.2)。 图3.2 Reporter方法检测 2’3’-cGAMP对 THP1-Dual细胞的抑制作用 3.2.2 TH...
产品名称: THP1-Dual? Cells .0 订购此产品 供应商: Invivogen 规格: 3-7 x 10e6 目录价: 16507.5 库存状态: 三周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: THP1-Dual™ Cells THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration...
THP-1 cell line: An in vitro cell model for immune modulation approach International Immunopharmacology Journal2014, International Immunopharmacology Wasaporn Chanput, ... Harry J. Wichers Highlights • THP-1 cell line is a suitable in vitro cell model to study modulation of monocyte and macropha...
The proliferative ability of cells in each group was detected by CCK-8 assay, and the cell cycle was detected by flow cytometry. TargetScan software was applied to predict the miR-126-3p target gene as RGS3, which was verified by dual luciferase reporter gene assay. The protein expression ...
上海玉博生物科技有限公司做为invivogen代理商之产品thpd-kostg-THP1-Dual KO-STING Cells 3-7 x 10e6 cells的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。
上海玉博生物科技有限公司做为invivogen代理商之产品thpd-r232-THP1-Dual™ KI-hSTING-R232 Cells 3-7 x 10e6 cells的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。
Required Cell Culture Media* Growth Medium: RPMI 1640, 2-4 mM L-glutamine, 10% (v/v) heat‑inactivated fetal bovine serum (FBS; 30 min at 56 ºC), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 mg/ml NormocinTMNotes:- The use of NormocinTM together with Pen-Strep is ...
See TDS for the exact contents of each cell line. 3-7 x 106 THP1-Dual™ hTLR7 cells OR THP1-Dual™ hTLR7 KO-TLR8 cells in a cryovial or shipping flask 1 ml of Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria,...