photoallergy ; THP-1 cell ; CD54 ; CD86 ; mean fluorescence intensity ; relative fluorescence intensity ; methodology validation [Abstract] Objective To identify and validate an in vitro photoallergy evaluation method with cell surface markers as research indicators. Methods THP-1 cells were incubate...
关键词: PMA;THP 1细胞; CD14;CD11b中图分类号: R733.7 文献标志码: A 文章编号: 1007-6611(2018)09-1051-04DOI:10.13753/j.issn.1007-6611.2018.09.010PMAinducestheexpressionofsurfacemarkersCD14andCD11bofTHP1celllineinhumanmonocyticleukemiaLIXiaoqin,CHENLirong(DepartmentofMedicalLaboratoryScience,Fenyang...
ELISA、流式细胞术等方法进行鉴定。（M1标记物如：CD80, iNOS, IL-1β, IL-6, CXCL1等；M2标记物...
图2 细胞表面标志CD11b、CD14表达Figure 2 Expression of cell surface markers CD11b and CD14 其次分别用10 ng/ml和50 ng/ml PMA处理THP-1细胞,发现正常培养条件下即对照组THP-1细胞CD14、CD11b表达量不高,而有报道称,PMA、TPA等可诱导未成熟的髓系细胞,向巨噬细胞方向分化,并使单核分化标志物CD11b和CD14...
比较不同诱导条件下的细胞THP-1细胞
C. (2009) Expression of surface markers on the human monocytic leukaemia cell line, THP-1, as in- dicators for the sensitizing potential of chemicals. Contact Dermatitis 60, 185-192.An S, Kim S, Huh Y, Lee TR, Kim H-K, Park K-L, Eun HC (2009) Expression of surface markers on ...
PMA诱导人单核细胞白血病细胞株THP-1细胞表面标志物CD14和CD11b的表达
Cell adherence, high phagocytic capacity and expression of differentiation-dependent cell surface markers; CD-14, CD36, TLR-2, and CR3 (CD11b/CD18), can be used to assure fully differentiation of THP-1 macrophage. 2.2 Advantages and disadvantages of THP-1 cell line Due to either financial ...
Progress on the development of human in vitro dendritic cell based assays for assessment of the sensitizing potential of a compound (THP-1, U-937, MUTZ-3, KG-1, HL-60, and K562) are extensively described along with biomarkers such as cell surface markers, cytokines, chemokines ... GGD Sa...
THP-1 and U937 are (pro-) monocytic cell lines that can, also in vitro, be differentiated into either various types of macrophages or into dendritic cells. This chapter describes how to grow THP-1, resp. U937 cells, how to differentiate these into more specialised phenotypes such as various...