ConclusionsmiR-126-3p may inhibit proliferation of human monocytic leukemia THP-1 cells and promote G 1-phase blockade by inhibiting the expression of RGS3. KEYWORDS: Leukemia, monocytic, acute;miRNA-126-3p;RGS3 protein, human;Cell proliferation;Cell cycle Corresponding author: Li Feng, Email:...
Naringenin enhanced curcumin‑induced apoptosis and cell viability inhibition. In addition, curcumin and naringenin induced cell cycle arrest at S phase and G2/M phase. Numerous pathways, including p53, c‑Jun N‑terminal kinases (JNK), Akt and extracellular signal‑regulated kinases (ERK)1...
[2]Andrographolide inhibits prostate cancer by targeting cell cycle regulators,CXCR3 and CXCR7 chemokine receptors[J].Hina Mir,Neeraj Kapur,Rajesh Singh,Guru Sonpavde,James W.Lillard,Shailesh Singh.Cell Cycle.2016(6) [3]CXCL12–CXCR7 axis is important for tumor endothelial cell angiogenic propert...
THP1-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin ®. SAFETY CONSIDERATIONS Biosafety Level 1 HANDLING PROCEDURES Required Cell Culture Medium • Growth Medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum (30 min at ...
bvCCK一8method.MorphologicalchangesofTHP一1cellstreatedwithbrucinewasdetectedbyacridineorange/ethidium brom i defA0l/EBdoublestaining.Annexin—V/PIdouble1abelingmethodwasusedtoassaytheapoptosisrateofTHP一1 cells.Theeffectofbrucineon HP.1cellcycledistributionwasdetectedbyPIsinglestaining.RT—PCRwasusedto ...
(CD34 CD38一cel1)inTHP一1,andthenthecellcycleofLSCsandnon—LSCswas compared.ThemRNAexpressionsofmuhidrugresistanceproteins(ABCG2,ABCB1)andapoptosisgene(Bcl2,Bax) wereexaminedbyrealtimequantitativePCRintwosubpopulations.NOD/SCIDmicewereinjectedwithdifferentsubpopu· lationsofTHP一1,andtheincidenceofleukemia...
Cell preparation 1. Centrifuge at 150 x g (RCF) for 10 mins or 300 x g (RCF) for 5 mins. 2. Remove supernatant and resuspend THP1-Dual™ hTLR9 cells at 5 x 105 cells/ml in freshly prepared, pre-warmed test medium. Detection of NF-κB induction 1. Add 20 µl of...
How ERK1/2 activation controls cell proliferation and cell death: Is subcellular localization the answer? [ J ]. Cell Cycle, 2009, 8(8): 1168-1175. Weston C R, Davis R J. The JNK signal transduction pathway [ J ]. Curr Opin Cell Biol, 2007, 19(2) : 142 - 149. 加载更多...
采用不同浓度壬基酚处理THP-1细胞48 h, Brdu实验检测壬基酚对THP-1巨噬细胞增殖的影响;b. Transwell实验检测壬基酚对THP-1巨噬细胞迁移的影响;c.迁移细胞数统计分析**p < 0.01) Fig. 2Effects of NP on cell proliferation and migration of THP-1 macrophages. (a. THP-1 macrophages were treated with...
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