结论 10ng/mlPMA能够诱导 THP 1细胞向巨噬细胞方向分化,为PMA诱导 THP 1细胞分化为巨噬细胞模型提供依据。关键词: PMA;THP 1细胞; CD14;CD11b中图分类号: R733.7 文献标志码: A 文章编号: 1007-6611(2018)09-1051-04DOI:10.13753/j.issn.1007-6611.2018.09.010PMAinducestheexpressionofsurfacemarkersCD14and...
结论LPS可导致THP一1细胞活化,显著上调CD14rnRNA的表达,并诱导产生炎症介质TNF-a和IL-6。关键词:脂多糖;CD14;肿瘤坏死因子;白细胞介素6;THP一1细胞中图分类号:R392TheEffectsofLPSonExpressionofmCD14inTHP.1CellLineHouBenxiang,ZhangChen,YinJun2(1.FacultyofStomatollogy,CapitalUniversityofMedicalSciences;2....
PMA诱导人单核细胞白血病细胞株THP-1细胞表面标志物CD14和CD11b的表达
expression wasenhanced谢th increasing concentrationsof LPS,andreachedthe highest valueas256timesasthecontrolat1 00ng/ml俨如.01);when5h, CDl4mRNA expression Was significantly decreasedinthe first,came tothelowestat 0.1 ng/ml as1/64asthe control衅0.01),and thenCD14mRNA expression increased withtheLPS...
Under this optimized conditions, the CD14 positive rate of THP-1 cells can reach 66.52%. Transcriptome sequencing showed that after the above induction, the mRNA expression of 3113 genes which were closely related to cell communication, signal transduction, cell response...
ultimately leading to the activation of NF-κB and the production of a pro-inflammatory response. Also, the TLR4 complex can be endocytosed into endosomes in a CD14-mediated fashion. This results in the the stimulation of IRF3 (interferon regulatory factor), which modulates the expression of typ...
Indeed, THP1-Dual™ cells do not respond to TLR7-specific ligands (see figures). Only the co-expression of TLR7 with its chaperone protein UNC93B1mut makes them responsive to TLR7-specific ligands (see figures). By additionally introducing a KO of endogenous TLR8, THP1-Dual™ hTLR7 ...
The expression of CD80 and CD86 on the THP⁃1⁃M1 cells were increased significantly, and CD163 and CD209 were highly expressed on the THP⁃ 1⁃M2 cells. For THP⁃1⁃DC cells, the expression of CD14 was significantly decreased, while the expression of CD80, CD86 [基金项目] ...
Exposing U937 monocytes to 20 ng/ml phorbol-12-myristate-13-acetate (PMA) for 72 h [10] or to 0.1 μmol vitamin D3 for 24 h [11] induced the expression of the CD14 macrophage marker gene. Inflammatory marker genes (TLR-4, TNF-α, NF-κB and its subunit genes p52, p50, RelB, ...