the use of a thiol-reactive resin can avoid false positives in streptavidin-based detection assays that can arise from endogenous proteins binding biotin.15Also, the specificity of a thiol-reactive resin in the SNO-RAC method can be immediately visualized or quantified by performing a control lacki...
After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution.doi:10.1016/S0021-9673(00)81791-4Trevor M. KitsonElsevier B.V.J ChromatogrPurification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B; Kitson, T. M...