S-nitrosothiol (SNO)-Resin Assisted Capture (SNO-RAC) relies on a Thiopropyl Sepharose resin to identify S-nitrosylated proteins (SNO-proteins) and sites of S-nitrosylation. Here, we present a protocol for preparing Thiopropyl Sepharose resin with efficiency of SNO-protein capture comparable to ...
Synonyms: Thiol Reactive Agarose Beads, Activated thiol Sepharose Thiopropyl Resin is used in purification of thiol group containing proteins, molecules and enzymes. The thiol-containing molecules are reversibly immobilized on the resin. When the sample to be purified is passed through the resin, thio...
Disulfiram-modified cytoplasmic aldehyde dehydrogenase binds covalently to the thiol groups of reduced thiopropyl-Sepharose 6B under conditions in which mitochondrial aldehyde dehydrogenase does not bind. After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution....