Taxanes such as DTX are antimitotic agents that work by inhibiting division of cells through unregulated formation of microtubules21. This has the added benefit of blocking the cells’ ability to go through mitosis, synchronizing the cells in the G2/M phase of the cell cycle, the most radio...
Transformed cells were recovered at 37 °C for 1 h and 100 μl was subsequently plated on an appropriate selective medium. Strain construction Strains were made using two separate methods. A complete list of strains and their deletion primers can be found in Supplementary Data 1. The ...
The growth curve shows a similar lag phase (until day 3) between the two lines analyzed. Following the lag phase (days 4–13), we noticed a significant discrepancy in the growth rate of CV compared to DS-CV. At day 6 the number of cells produced by CV was almost twice that observed...
You may recall that during the S phase each chromosome is duplicated, forming a new chromosome with two sister chromatids, which become visible microscopically early in the M phase. The key function of mitosis is to separate the sister chromatids and deliver them to the two daughter cells, ...
One difference between cell division in plants and animals is that plant cells form a cell wall after mitosis to separate the nuclei and cytoplasm of the two new identical cells. After animal cells undergo mitosis, the cell membrane pinches together alon
In cells, ATG9 is suggested to reside in the acceptor membrane, while other scramblases reside on the donor membrane on the ER.47 To test the effect of adding scramblases to membranes, we measured lipid transfer by ATG2 between two ATG9-containing PLs. Using an established assay, donor...
“This algorithm enables us to create precise DNA tools for marking and studying cells, offering new insights into cellular behaviors,” says Gargiulo, senior author of the study. “We hope this research opens doors to a more straightforward and scalable way of understanding and manipulating cells...
Along the way, you'll see chemical reactions first-hand and meet entertaining characters like Enzyme Man and Drinkzilla, who show how the liver metabolizes alcohol. Together with Ami and Rin, you'll learn all about: * The organelles and proteins inside cells, and how they support cellular ...
doi:10.1038/bjc.1962.15StoneDavidBritish Journal of CancerSTONE D. The use of amitotic cells for the study of factors involved in mitosis: the possible involvement of DNA, RNA and unsaturated fatty acids. Br J Cancer. 1962 Mar; 16 :145–156....
At the initial stage of carcinogenesis single mutated cells appear within an epithelium. Mammalian in vitro experiments show that potentially cancerous cells undergo live apical extrusion from normal monolayers. However, the mechanism underlying this pro