Boxplot width is proportional to the square-roots of the number of cells in each group. d Subcellular distribution of viral 4i markers in HeLa cells at 12 hpi. For each marker, a zoom-in image of an example cell is shown next to the composite image (dashed-line). Nucleus and cell ...
Cells were washed with phosphate-buffered saline (PBS), harvested and RNA extracted (RNeasy Mini, Qiagen, Germany). RNA was converted to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Variant screening of NEK1 in an OFD cohort To evaluate whether NEK1 variants ...
Human Immunodeficiency Virus Type 1 DNA Nuclear Import and Integration Are Mitosis Independent in Cycling Cellsdoi:10.1128/JVI.77.24.13412-13417.2003Richard A KatzJames G GregerPamela BoimelAnna SkalkaKatz RA, Greger JG, Boimel P and Skalka AM (2003) Human immunodeficiency virus type 1 DNA ...
First, we developed, applied, and benchmarked 3DeFDR-5C using 5C data across three distinct cellular states: mouse embryonic stem (ES) cells cultured in 2i media representing a naive pluripotent state, mouse ES cells cultured in LIF/serum representing the primed pluripotency state, and primary ...
Protein kinases and phosphatases regulate protein phosphorylation, a critical means of modulating protein function, stability and localization. The identification of functional networks for protein phosphatases has been slow due to their redundant nature
Protein kinases and phosphatases regulate protein phosphorylation, a critical means of modulating protein function, stability and localization. The identification of functional networks for protein phosphatases has been slow due to their redundant nature
in individual cells, and expression levels of individual genes within each cell can then be determined. This approach enables the expression profiles of thousands of individual cells to be analyzed, and the cells can then be grouped (clustered) based on their similarities. The groups of cells ...
Moreover, extensive transcriptional comparisons between reactivated astrocytes and oligodendrocyte lineage cells in response to cerebral ischemia are still lacking. Here we present a large-scale single-nucleus transcriptome dataset of the brain´s acute response to ischemic stroke. We dissect subtype-...
The present disclosure relates to polypeptides, such as fibronectin type III (FN3) domains that can bind CD71, their conjugates, isolated nucleotides encoding the molecules, vectors, host-cells, as well as methods of making and using the same.Inventors...
Fibronectin type III domains (FN3) that specifically bind to CD8A, related polynucleotides capable of encoding CD8A-specific FN3 domains, cells expressing the FN3 domains, as well a