Because the SRY gene is found in all nucleated cells of males only, whereas the [beta]-globin gene is present in all nucleated cells of both males and females (6), we calculated the percentage of male DNA in a particular plasma or buffy coat sample, denoted as Y%, using the following...
SRY was identified in an attempt to clone the mammalian testisdetermining factor. Evidence in the form of sex-reversing mutations and mouse transgenesis experiments indicates that SRY is the testis-determining factor. The cloning of this gene marks the end of the beginning of our understanding of ...
Changes in technology in the past decade have had such an impact on the way that molecular evolution research is done that it is difficult now to imagine working in a world without genomics or the Internet. In 1992, GenBank was less than a hundredth of i
The mammalian sex determining gene, sex region Y chromosome (SRY), is now firmly established as the testis determining locus. The SRY locus is close to the short arm Y terminus and just distal to zinc finger Y region (ZFY), a locus previously thought to be involved in testicular differentia...
Circular transcripts of the testis-determining gene Sry in adult mouse testis. Cell 73, 1019–1030 (1993). Article CAS PubMed Google Scholar Wang, P. L. et al. Circular RNA is expressed across the eukaryotic tree of life. PLOS ONE 9, e90859 (2014). Article CAS PubMed PubMed Central...
Here, we disrupted the mouse Spo11 gene and determined it is essential for gametogenesis and meiotic chromosome synapsis. In addition, we found that Spo11 localizes to discrete foci early in meiosis and later to regions of homologous chromosome synapsis. Furthermore, Spo11 expression and localizati...
METHODS: Transgenic NZM2328 mice were created by deletion of the Sry gene from the Y chromosome, thereby separating genetic from gonadal sex. Survival, renal histopathology and markers of immune activation were compared in mice carrying the XX versus the XY(-) sex chromosome complement, with each...
The scientists believe that this extra piece of DNA is like a switch that turns on SOX9, even without the SRY gene. To test their idea, they put this extra DNA into mice and found out that it made SOX9 work better. This means that even without SRY, SOX9 can still do its job, thank...
The molecular basis underlying the three Y(DOM) classes is an enigma. The simplest explanation is that they harbor different alleles of the testis-determining gene, Sry. Sequencing of Sry(DOM) genes has indeed identified polymorphisms. However, none were unequivocally linked to the sex-reversal ...
Reference 46 shows that a Y-chromosome-linked duplicate of the Dmrt1 gene (namely, Dmy ) is required for male sex determination in medaka and, along with reference 47, shows that this gene functions analogously to Sry in mammals. Article CAS PubMed PubMed Central Google Scholar Wang, D....