Introduction The Protein Processing Assembly Line Polypeptide Production The Endoplasmic Reticulum: Polypeptide Folding and Modification Overview of the ER Lumen Folding in the ER Glycosylation in the ER Glycosylphosphatidylinositol-Linked Proteins Membrane Traffic: ER to Golgi and Beyond The Golgi Apparatus ...
we first sought to crystallize VenA in its substrate-free form. However, we did not obtain any crystals of the wild-type VenA (VenAWT) and supposed that theN-terminal flexible loop region (1-15 aa) might prevent the protein crystallization. Thus, the truncated form VenAΔ1-15with theN...
1a). The 9-1-1 clamp loading process is assisted by replication protein A (RPA), which covers the ssDNA adjacent to the 5′-recessed DNA junction19. The structures of the yeast and human RFC–PCNA complexes in the absence of DNA show that each Rfc subunit contains the AAA+ (ATPases ...
prion protein prion disease protein self-assembly aggregation intrinsically disordered protein Abbreviations DLS dynamic light scattering FRAP fluorescence recovery after photobleaching LLPS liquid–liquid phase separation MBP maltose-binding protein PrP prion protein PrPC cellular prion protein TEV tobacco etch ...
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1–5. Here we describe a general solution to this problem that starts
Alternative splicing (AS) is a principal mode of genetic regulation and one of the most widely used mechanisms to generate structurally and functionally distinct mRNA and protein variants. Dysregulation of AS may result in aberrant transcription and protein products, leading to the emergence of human...
Post-translational modifications (PTMs) regulate various aspects of protein function, including degradation. Mass spectrometric methods relying on pulsed metabolic labeling are popular to quantify turnover rates on a proteome-wide scale. Such data have t
A. et al. Structural basis of tail-anchored membrane protein biogenesis by the GET insertase complex. Mol. Cell 80, 72–86 e77 (2020). Cryo-EM structures of the yeast and human GET1–GET2–GET3 complexes reveal a heterotetrameric membrane assembly with hydrophilic vestibules into the ...
An increase in hydrophilicity leads to an increase in protein adsorption and reduces scaffold toxicity, which plays a crucial role in cell attachment. The interaction between cells and components of the extracellular matrix (ECM) such as fibronectin, vitronectin, collagen, and laminin can impact cell...
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